OJ手性柱分离芳基烷酸类非甾体解热镇痛药的色谱保留特性和拆分机理  

Retention Characteristics and Separation Mechanism of Enantiomers of Arylpropionic Acid Nonsteroidal Antiinflammatory Drugs with OJ Column

作　　者：余勤[1] 南峰[1] 向瑾[1] 梁茂植[1] 秦永平[1] 

机构地区：[1]四川大学华西医院临床药理研究室,成都610041

出　　处：《化学学报》2008年第9期1079-1085,共7页Acta Chimica Sinica

摘　　要：使用纤维素类手性柱OJ柱,分离布洛芬、依托度酸、非诺洛芬钙、酮洛芬、洛索洛芬等5个芳基烷酸类非甾体解热镇痛药对映体,并通过溶质计量置换保留模型和热力学研究对OJ手性柱手性分离机理进行探讨.结果表明,OJ手性柱可在正相条件下分离系列芳基烷酸类非甾体解热镇痛药,对映体的色谱保留和分离度可以通过改变流动相中醇类置换剂和浓度、有机酸性改性剂和浓度、柱温等因素调节.Five arylpropionic acid nonsteroidal antiinflammatory drugs, i.e. ibuprofen, etodolac, fenoprofen calcium salt hydrate, ketoprofen and loxoprofen, were enantiomerically separated on a commercially available OJ cellulose chiral stationary phase by high performance liquid chromatography in a normal mode. The mechanism of enantioseparation was studied by stoichiometric displacement model for retention and thermodynamic study. It was found that the chromatographic retention and enantioseparation could be generated by the type of alcohol displacer and organic acid modifier, modification of the concentration of alcohol displacer and organic acid modifier, and change of column temperature.

关 键 词：高效液相色谱法 手性固定相 对映体分离 非甾体解热镇痛药 

分 类 号：R917[医药卫生—药物分析学]