促性腺激素释放激素对大鼠胰腺细胞内cAMP含量的影响  

Effects of stimulators and blockers of GnRH receptors on cAMP production by rat pancreatic cells in vitro

作　　者：曹宏伟[1] 蒲若蕾[1] 黄威权[2] 姚兵[2] 陈蕾[2] 张荣庆[3] 姬秋和[1] 

机构地区：[1]第四军医大学西京医院内分泌科,陕西西安710033 [2]第四军医大学基础部组胚教研室,陕西西安710033 [3]清华大学生物系,北京100084

出　　处：《第四军医大学学报》2008年第9期813-815,共3页Journal of the Fourth Military Medical University

摘　　要：目的：通过观察GnRH类似物（GnRHR激动剂和拮抗剂）对大鼠胰腺细胞内cAMP含量的影响,了解cAMP在GnRHR介导的信号转导通路中的作用.方法：以不同浓度GnRH类似物对原代培养的大鼠胰腺细胞进行刺激,借助放免法测定观察在体外条件下GnRH类似物对大鼠胰腺细胞内cAMP含量的影响.结果：在量效实验中,各浓度GnRHR激动剂均能使细胞内cAMP含量增加,其中10^-8～10^-5mol/L中4个浓度组最为明显,与对照组相比有非常显著差异（P〈0.01）.应用GnRHR拮抗剂（10^-6mol/L）后明显抑制了激动剂组增高cAMP含量的作用,其cAMP水平与对照组比较没有显著差异（P〉0.05）.在时效实验中,应用10^-7mol/LGn-RHR激动剂刺激细胞,从10min起细胞内cAMP含量即开始增多,到1h达到峰值,以后逐渐下降,8h后恢复到原水平.结论：由以上结果推测cAMP可能是GnRHR介导的信号转导通路中的信号分子之一.AIM： To observe the effects of stimulators and blockers of gonadotropin releasing hormone （GnRH） receptors on cAMP production by rat pancreatic cells in vitro. METHODS： Cell culture and radioimmunoassay were used to investigate the changes of cAMP production in culture media supplemented with different concentrations of GnRH analogues. RESULTS： The Gn- RH agonist could stimulate cAMP production in a concentration- dependent manner from 10^-8 to 10^-5 mol/L, which was blocked by the GnRH antagonist（ 10^ -6 mol/L,P 〈0.01 ）. The cAMP production could be stimulated by the GnRH agonist（ 10 ^-6 mol/L） in a time-dependent manner, which reached its peak at 1 h after the stimulation and returned to its original level at 8 h later. CONCLUSION： cAMP may be one of the signaling factors involved in the GnRH receptor＇s signaling pathway.

关 键 词：大鼠 胰腺 促性腺激素释放激素受体 信号转导 环磷酸腺苷 

分 类 号：R977.1[医药卫生—药品]