ICP-MS法在测定转基因酵母中锌和铁含量的应用  

The Application of ICP-MS in Investigating Content of Metal Ion Elements in Transgenic Yeast

作　　者：冯长庚[1] 张放[1] 刘翠梅[2] 施燕支[2] 黄勤妮[1] 印莉萍[1] 

机构地区：[1]首都师范大学植物功能基因组实验室,北京100037 [2]首都师范大学分析测试中心,北京100037

出　　处：《现代科学仪器》2008年第2期69-71,共3页Modern Scientific Instruments

基　　金：国家自然科学基金(30770178);北京市自然科学基金(5042004);北京市教委科技发展基金重点(K7200710028013)。

摘　　要：采用电感耦合等离子体质谱法测定了不同转基因型酵母细胞中锌和铁含量,此方法测定结果与酵母异源功能互补实验得到的结果一致。通过ICP-MS与异源互补的对比分析结果,证明了MxIRT1(小金海棠铁转运基因)基因具有转运金属离子的功能,其作为非特异的金属离子转运体在细胞质膜上发挥作用,对Zn,Fe都有显著的吸收。ICP -MS法的优点是其测定数据可以直接呈现出酵母对金属元素吸收的趋势。A method was studied for the genic yeast by using ICP - MS. The result was analysis of several kinds of metal ion elements （Fe, Zn） in transin consistent with the result by using functional complementation of yeast mutant strains. Combined with the two methods, it can be proved that MxIRT1 gene has a function of transporting metal ions. MxIRT1, as a nonspecific metal ion transporter which has an obvious absorption to zinc and iron, plays a role in cell membrane. And by using the ICP- MS method, the trend of metal ion absorption in yeast can be seen directly in quantity.

关 键 词：ICP-MS 转基因酵母 IRT(调节铁转运的蛋白) 

分 类 号：Q78[生物学—分子生物学]