体细胞重编程为多潜能干细胞的研究进展  

Studies of somatic cells epigenetic reprogramming to pluripotent stem cells

作　　者：徐燕宁[1] 关娜[1] 张庆华[1] 雷蕾[1] 

机构地区：[1]哈尔滨医科大学组织学与胚胎学教研室,哈尔滨150081

出　　处：《生命科学》2008年第2期231-236,共6页Chinese Bulletin of Life Sciences

基　　金：国家自然科学基金(30671025);黑龙江省教育厅海外学人科研资助项目(1151hz031);哈尔滨医科大学归国留学科研启动基金

摘　　要：人类的胚胎干细胞(embryonicstemcells,EScells)可以用来治疗很多疾病,但是如果通过核移植来获得与供体或者患者相匹配的ES细胞,就会受到人卵母细胞来源等条件的制约。这就促使了将体细胞重编程为多潜能细胞这样一种技术策略的发展,其中包括将分化细胞与ES细胞融合,在卵细胞、ES细胞或多潜能癌细胞的抽提物中孵育,强制多潜能因子过表达等具体的方法。通过这些途径引出了一些核功能的重编程以及相应的DNA甲基化修饰、组蛋白翻译后修饰,使体细胞表达特定的多潜能因子,转变为类似胚胎干细胞的多潜能细胞。Nuclear transplantation remains to date the method of choice for creating donor- or patient-matched ES cells, however, the scarce of human oocyte triggered the development of alternative strategies for reprogramming cells to a pluripotent cell, including fusion of differentiated cells with ESCs, incubation of cells in extracts of eggs, ESCs, or pluripotent carcinoma cells and by forced overexpression of pluripotency factors. These approaches elicit at least some reprogramming of nuclear function and accordingly modification patterns of DNA methylation and histone post-translation. Overexpression of defined pluripotency factors in somatic cells leads to transforming these ceils to pluripotent ES-cell-like state.

关 键 词：体细胞 重编程 多潜能干细胞 细胞治疗 

分 类 号：Q813[生物学—生物工程]