二色补血草硫氧还蛋白(Trx)的克隆和表达分析  

Cloning and Expression Analysis of Thioredoxin Gene (Trx) from Limonium bicolor

作　　者：张大伟[1] 杨传平[1] 王玉成[1] 班巧英[1] 马辉[1] 

机构地区：[1]东北林业大学林木遗传育种与生物技术教育部重点实验室,哈尔滨150040

出　　处：《植物生理学通讯》2008年第2期201-205,共5页Plant Physiology Communications

基　　金：教育部科学技术研究重点项目(107037);国家自然科学基金面上项目(30571509);黑龙江省攻关重点项目(GB068303-1)

摘　　要：从二色补血草cDNA文库中分离出1个硫氧还蛋白基因全长cDNA序列。基因全长1138 bp,其中,5′非翻译(UTR)区128 bp,3′非翻译区212 bp,开放阅读框(ORF)全长798 bp,编码265个氨基酸,编码蛋白的分子量为28.58 kDa,理论等电点(pI)为9.68。BlastP分析表明二色补血草Trx与拟南芥Trx序列同源性为52%,与葡萄Trx序列同源性为76%,从11个物种的氨基酸多序列比对可以看出Trx氨基酸序列保守性较高。实时定量RT-PCR方法检测低温、NaCl和PEG胁迫不同时间后的基因在二色补血草中表达模式的结果表明,NaCl能诱导Trx基因在二色补血草叶中表达,胁迫24 h后达到高峰,而聚乙二醇和低温处理则抑制Trx在二色补血草根和叶的表达.The full length cDNA of a novel thioredoxin was cloned from Limonium bicolor cDNA library. The sequence of thioredoxin was 1 138 bp in length, including 128 bp of 5＇ untranslated region and 212 bp of 3＇ untran-slated region. It had an open reading frame （ORF） of 798 bp and encoded a protein with 265 amino acid residues. The encoded protein molecular weight was 28.58 kDa and its theoretical pI was 9.68. Multiple sequence alignment of Trx proteins from 11 plants revealed that the Trx gene share high identifies in sequence.We examined the expression pattern of the Trx gene in leaf and root of L. bicolor treated with NaCl, low temperature and PEG stresses for differenr time using real time RT-PCR. The results showed that the Trx was induced by NaCl treatments in leaf of L. bicolor with a expression peak at treatment for 24 h. However, Trx was inhibited by low temperature and PEG treatment in both leaf and root of L. bicolor.

关 键 词：二色补血草 硫氧还蛋白 基因克隆 实时荧光定量PCR 

分 类 号：Q943.2[生物学—植物学]