盐胁迫下青钱柳幼苗叶片中H^＋-ATP酶的细胞化学定位和超微结构变化  

Cytochemical Localization of the H^+-ATPase and Variation of Ultra-Structure in Leaves of Cyclocarya Paliurus Seedlings under Salt Stress

作　　者：姚瑞玲[1] 王胤[1] 方升佐[2] 

机构地区：[1]广西林业科学研究院,南宁530001 [2]南京林业大学森林资源与环境学院,南京210037

出　　处：《植物生理学通讯》2008年第2期206-210,共5页Plant Physiology Communications

基　　金：江苏省高新技术研究计划(BG2006314);国家自然科学基金(30371156)

摘　　要：用透射电镜观察盐胁迫下3种青钱柳幼苗叶中H^+-ATPase活性的细胞化学定位及其超微结构的结果表明,正常生长情况下,幼苗叶中H^+-ATP酶活性较小,且多分布于细胞核上。盐处理12 d后,随着盐浓度的增加,H^+-ATPase活性越大,盐胁迫20 d的叶中亚细胞结构受破坏的程度越小,幼苗盐适应能力越强。H^+-ATPase主要分布在细胞核中的显示其受盐害的程度较轻,主要分布在液泡中的显示其受盐害的程度较重。从整体上看,3种青钱柳幼苗的耐盐能力大小为:安徽黄山幼苗>云南昆明幼苗>江西九江幼苗。By adopting electron transmission microscopy to observe the cytochemical localization of H^＋-ATPase activities and the variation of ultra-structure in leaves of Cyclocarya paliurus seedlings for three kinds, the results showed that under normal condition, H＋~ATPase activities were lower and it mainly located in nuclear. After 12 d of salt treatment, with the increase of salinity, the greater activities of H^＋-ATPase were, the slighter the damaged degree that leaf subcell suffering after 20 d of salt stress was and the stronger the salt-adaptation capacity of seedlings was. When the H^＋-ATPase mainly located on nucleus, this showed that the salt-damage degree of seedlings was slighter; however, when it mainly located on vacuoles, this indicated that the salto damage degree of see^tlings was more serious. Generally, salt-tolerance capacities of C. paliurus seedlings for three kinds were in the order of Huangshan seedlings from Anhui 〉Kunming seedlings from Yunnan 〉Jiujiang seedlings from Jiangxi.

关 键 词：盐胁迫 青钱柳 H^+-ATP酶 亚细胞化学定位 超微结构 

分 类 号：Q945[生物学—植物学]