细胞表面糖链类型鉴定及其与副粘病毒结合的特异性  

Binding of paramyxoviruses to gangliosides on the surface of Vero cells

作　　者：宋战昀[1] 冯新[1] 韩文瑜[1] 刘伟[1] 刘影波[1] 丁壮[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062

出　　处：《中国兽医学报》2008年第1期24-27,共4页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30571375)

摘　　要：通过糖链检测试剂盒对Vero细胞进行细胞凝集素组织化学染色鉴定,结果显示,Vero细胞与凝集素MAA和SNA呈强阳性反应,而与凝集素GNA和PNA呈阴性反应,表明Vero细胞表面具有唾液酸α2-3连接的半乳糖和唾液酸α2-6连接的半乳糖。Vero细胞神经节苷脂提取及纯化结果显示,GD1a是主要神经节苷脂组分,同时还存在GM1、GM2和GM3。为进一步确定神经节苷脂在病毒入侵中的作用和意义,使用神经节苷脂标准品及Vero细胞神经节苷脂提取物对新城疫病毒和鹅副粘病毒分别进行红细胞吸附抑制试验,结果表明,2种病毒与不同类型神经节苷脂结合特异性上存在差异。Gangliosides on the cell surface can act as viral receptors,but it is unclear ff the species and relative abundance of these can affect viral entry. Immunohistochemical analyses of Vero cells, by using the DIG Glycan Differentiation Kit （ Roche Molecular Diagnostics） ,showed that Veto cells reacted with lectins MAA and SNA but not with GNA and PNA,indicating that the cell surface contains sialic acid linked a2-3 and a2-6 to galactose. Analysis showed that GD1a was the predominant ganglioside component, with GM1, GM2 and GM3 also being detected. Gangliosides from Vero cells and individual gangliosides were used in hemadsorption inhibition assays to test their ability to inhibit the binding of NDV and Goose paramyxovirus. Results showed that the two viruses bound specifically to different gangliosides.

关 键 词：VERO细胞 神经节苷脂 副粘病毒 病毒入侵 

分 类 号：S852.65[农业科学—基础兽医学]