甘露醇和β-七叶皂苷钠致兔耳静脉和周围组织损伤的研究  

Studies on damage of auricular vein and peripheral tissue caused by mannitol and β-aescine sodium in rabbits

作　　者：王惠冰[1] 张晋萍[2] 

机构地区：[1]南京大学附属鼓楼医院骨科关节中心,南京210008 [2]南京大学附属鼓楼医院药剂科,南京210008

出　　处：《药物不良反应杂志》2008年第2期86-88,共3页Adverse Drug Reactions Journal

摘　　要：目的：比较甘露醇和β-七叶皂苷钠所致兔耳静脉及周围组织的损伤。方法：将健康家兔12只，随机分为3组，每组4只。甘露醇组静脉注射20％甘露醇5ml；β-七叶皂苷钠组静脉注射0．02％β-七叶皂苷钠5ml；对照组静脉注射0．9％氯化钠注射液5ml。每组均注射2次／d，上午注射左耳缘静脉，下午注射右耳缘静脉，共注射3d。分别观察各组在第1天、第3天兔耳缘静脉血管和周围组织的损伤以及第4～7天时各自的恢复程度。结果：病理切片显示：第1天时，甘露醇组及对照组兔耳血管和周围组织无明显改变；而β-七叶皂苷钠组兔耳血管明显扩张，出血。第3天时，在注射部位0．5cm范围内，甘露醇组和β-七叶皂苷钠组兔耳血管及周围组织的损伤均有加重：甘露醇组为血管内皮细胞破坏，血管内瘀血，周围组织炎症水肿；β-七叶皂苷钠组血管扩张及炎症反应。对照组无明显变化。第4天，甘露醇和β-七叶皂苷钠组周围组织炎症及水肿各有不同程度的恢复；第5天和第7天，甘露醇组出现2例在距离注射部位约2cm和3．5cm处，一块约1cm×1cm、另一块约0．6cm×0．4cm的局部组织坏死；而β-七叶皂苷钠无1例发生局部组织坏死。结论：甘露醇致血管出现损伤的时间较β-七叶皂苷钠延迟，但对血管和周围组织产生损伤的程度较β-七叶皂苷钠重。Objective： To compare the damage of auricular vein and peripheral tissue caused by mannitol and β-aescine sodium in rabbits. Methods： Twelve healthy rabbits were randomly divided into three groups with 4 rabbits in each group： the mannitol group received 5 ml of mannitol 20% intravenously, the β-aescine sodium group received 5 ml of aescine sodium 0.02% intravenously, the control group received 5 ml of sodium chloride 0.9% intravenously. Each rabbit received two injections every day, ie, the left ear margin vein received the injection in the morning, and the right ear margin vein received the injection in the afternoon. The duration of medication was 3 days. The severity of damage on day 1 and day 3 and the degree of recovery on day 4 ～ 7 to the rabbits＇ auricular vein and peripheral tissue were observed. Results： A pathological examination showed that on day 1, no marked changes in vascular and peripheral tissue of rabbit＇s ears were found in the mannitol and control groups, but vascular expansion and hemorrhage of rabbit＇s ears were found in the β-aescine sodium group. On day 3, the damage to vascular and peripheral tissue of rabbit＇s ears which were within 0. 5 cm of the injection site was aggravated in the mannitol and β-aescine groups as follows： distruction of vascular endothelial cells, intravascular stasis and inflammatory edema of peripheral tissue occurred in the mannitol group; vascular expansion and inflammatory response occurred in the β-aescine sodium group; and no changes were found in the control group. On day 4, inflammation and edema to the peripheral tissue relieved in varying degrees in the mannitol and β-aescine sodium groups. However, on day 5 and day 7, a 1 cm × 1 cm nercotic tissue which was 2 cm from the injection site, and a 0.6 cm × 0.4 cm necrotic tissue which was 3.5 cm from the injection site occurred in two rabbits of the mannitol group, respectively. No necrotic tissue was found in the β-aescine sodium group. Conclusion： The appearance of damage caus

关 键 词：甘露醇 Β-七叶皂苷钠 耳静脉 周围组织 损伤 兔 

分 类 号：R96[医药卫生—药理学]