我国6个弓形虫虫株线粒体cox1部分序列的比较分析  

Comparative Analysis of DNA Sequences of Mitochondrial Cytochrome c oxidase Subunit 1 Among Six Toxoplasma gondii Strains from China

作　　者：周东辉[1] 钟柳艳[1] 张翰[1] 林瑞庆[1] 宋慧群[1] 朱兴全[1] 

机构地区：[1]华南农业大学兽医学院,广州510642

出　　处：《热带医学杂志》2008年第4期299-301,共3页Journal of Tropical Medicine

基　　金：国家"973"计划前期研究专项资助课题(No.2007CB116301);教育部"长江学者和创新团队发展计划"创新团队项目(No.IRT0723);广东省高等学校自然科学研究重点项目(No.06Z004)。

摘　　要：目的对采自我国的6个弓形虫虫株线粒体细胞色素c氧化酶第Ⅰ亚基(cox1)基因部分序列(pcox1)进行比较分析,并与RH株的相应序列进行比较,研究弓形虫线粒体cox1基因与弓形虫的种群遗传关系。方法应用聚合酶链反应(PCR)扩增弓形虫不同虫株的pcox1,并进行序列分析比较。结果每个虫株都获得599bp的pcox1序列,比较分析表明,弓形虫不同虫株的pcox1序列之间没有差异。结论弓形虫pcox1序列不能作为种群遗传变异研究的标记,也与弓形虫毒力无关。研究结果为进一步研究弓形虫的群体遗传结构奠定了一定的基础。Objective To perform a comparative analysis of cytochrome c oxidase subunit 1 （cox1） gene of various strains of Toxoplasma gondii, and to study the relationship between the cox1 gene and the genetic structure of Toxoplasma, Method Partial cox1 （pcox1） was amplified from each strain of T.gondii and the amplicon was analyzed by sequencing, Result Sequence comparison revealed that there was no difference in the nucleotides of the pcox1 sequences obtained from various strains of T.gondii. Conclusion pcox1 sequence can not be used as a genetic marker for the population genetic studies, and it is also not related to the virulence of T.gondii, Results from the present study provide foundation for the future studies of population genetics of T.gondii.

关 键 词：弓形虫 线粒体DNA cox1 PCR 序列分析 

分 类 号：R382.5[医药卫生—医学寄生虫学]