热处理和紫外辐射对DNA影响的拉曼光谱研究  

Raman Spectroscopic Study on the Influence of Heat Treatment and Ultraviolet Radiation on DNA

作　　者：柯惟中[1] 余多慰[1] 陈婉蓉[1] 缪丽萍[1] 

机构地区：[1]南京师范大学理化中心江苏省光电技术重点实验室

出　　处：《光学学报》1997年第12期1681-1686,共6页Acta Optica Sinica

摘　　要：检测了鲱鱼精ＤＮＡ纤维和经过４０℃、９１℃、２００℃加热处理的拉曼光谱。研究结果表明，在熔融温度以下热处理对ＤＮＡ构象的影响是轻微的，在熔融温度以上则随温度的升高，对ＤＮＡ分子结构的影响及破坏也逐渐加剧，首先受影响的是腺嘌呤和脱氧核糖。对天然鲱鱼精ＤＮＡ溶液和经紫外光照射后溶液拉曼谱图的检测分析表明，紫外辐射会严重影响ＤＮＡ的构象，损伤碱基间的氢键和基团，所造成ＤＮＡ的变性是不可逆的。The Raman spectra of berring sperm DNA in antive fibers and after heat treatments to 40℃, 91℃, and 200℃ were presented. The Raman spectral bands indicated that the damage of the heating on the DNA conformation is limited below the melting point. The damage of the DNA molecular structure increased with a rise in temperature above the melting point. The damage was first obversed in adenine and deoxyribose. The Raman spectra of DNA in aqueous solution and after ultraviolet radiation were reported. The experimental results proved that ultraviolet radiation had a serious influence on the DNA molecular conformation and damaged the hydrogen bonds and groups among the purine and pyrimidine bases. The denuturation was irreversible.

关 键 词：拉曼光谱 脱氧核糖核酸 热处理 紫外辐射 

分 类 号：Q523.02[生物学—生物化学] Q691.2