NMDA谷氨酸受体NR1亚基在大鼠前庭终器的表达  

Expression of NR1 glutamate receptor subunit mRNA in the rat vestibular end-organs

作　　者：贺海霞[1] 孔维佳[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院耳鼻咽喉科,武汉430022

出　　处：《中华耳科学杂志》2007年第4期442-445,共4页Chinese Journal of Otology

基　　金：国家杰出青年基金资助项目(NO:39925035);卫生部临床学科重点项目(NO:2004);国家自然科学基金(NO:30371525);国家科技部"十五"攻关计划(NO:2004BA720A18-02);教育部高等学校博士学科点专项科研基金(NO:2002173)。

摘　　要：目的研究NMDA谷氨酸受体NR1亚基在大鼠前庭终器的表达。方法运用RT-PCR技术检测大鼠前庭终器NR1亚基的基因表达。以大鼠脑组织RNA为阳性对照。结果在前庭终器中扩增出标志NMDA谷氨酸受体NR1亚基基因表达的PCR产物,并经测序证实与预计扩增的cDNA片断序列一致。结论NMDA谷氨酸受体NR1亚基在大鼠前庭终器有表达,为谷氨酸作为前庭重要的神经递质之一提供了证据。NMDA谷氨酸受体可能作为自身受体在前庭系统神经信息传递过程中起到正反馈调节作用。Objective To investigate the expression of the NR1 glutamate receptor subunit mRNA in the rat inner ear vestibular end-organs. Methods Using a combination of reverse transcription followed by polymerase chain reaction （RT-PCR）, expression of the NR1 glutamate receptor subunit mRNA in the rat inner ear vestibular end-organs was examined. The brain RNA was used as positive control. Results The NR1 glutamate receptor subunit mRNA was expressed in the rat vestibular end-organs by means of a RT-PCR assay and sequence analysis. Conclusion The result indicates that glutamate is one of the important neurotransmitters in the vestibular system, and NMDA glutamate receptor may plays a role as autoreceptor in the neurochemical transmission in the vestibular system.

关 键 词：NMDA谷氨酸受体NR1 前庭 逆转录-聚合酶链反应技术 大鼠 

分 类 号：R339.16[医药卫生—人体生理学] R349.82[医药卫生—基础医学]