黄芪多糖对糖尿病KKAy小鼠肝脏脂肪变性的影响  

Ameliorative effect of astragalus polysaccharide (APS) on the hepatic steatosis in diabetic KKAy mice

作　　者：毛先晴[1] 欧阳静萍[1] 吴柯[1] 刘敏[1] 吴勇[1] 徐怡[1] 

机构地区：[1]武汉大学医学院病理生理教研室、湖北省过敏与免疫相关疾病重点实验室,430079

出　　处：《中国糖尿病杂志》2008年第4期233-236,共4页Chinese Journal of Diabetes

基　　金：国家自然科学基金资助项目(303705673)

摘　　要：目的探讨黄芪多糖(APS)改善糖尿病KKAy小鼠肝脏脂肪变性的作用及机制。方法KKAy小鼠16只,随机分为糖尿病对照(KK)组8只和APS治疗(KA)组8只。C57BL/6J小鼠20只,随机分为正常对照(NC)组10只和APS对照(CA)组10只。测血液及肝脏生化指标;判定胰岛素抵抗程度;观察肝脏病理学改变;检测GSK3β和胰岛素诱导的ser9GSK3β表达。结果治疗8周后,KA组血糖降低,胰岛素敏感性增强;肝TG,FFA含量减少,肝脂肪变性减轻;肝GSK3β表达减少,ser9GSK3β表达增加(P<0.05)。结论APS可以改善糖尿病KKAy小鼠肝脏脂肪变性,其机制与减少高糖高脂的肝毒性和GSK3β的表达及活性,改善胰岛素抵抗有关。Objective To investigate the ameliorative effect and a mechanism of APS on the hepatic steatosis in diabetic KKAy mice. Methods KKAy and C57BL/6J mice were respectively seperated into KK and K＋ A（n= 8）as well as C and C＋ A（n= 10）groups. Blood and hepatic biochemical parameters were observed. Insulin sensitivity was analyzed by OGTT ＆ HOMA-IR. Hepatic pathological changes were presented through microscope and TEM. The expressions of hepatic GSK3β and insulin-induced Ser9GSK3β were performed by Western blot. Results With 8-week APS therapy in K＋A group,the levels of blood glucose and hepatic TG and FFA were decreased, insulin sensitivity was improved,the hepatic steatosis was significantly alleviated （P〈0.05）,the expression of hepatic GSK3β was decreased and Ser9GSK3β expression was increased（all P〈0. 05）（P〈0. 05）. Conclusions APS can ameliorate the hepatic steatosis in diabetic KKAy mice. It may correlate with lessening the gluco-toxicity and lipo-toxicity to liver and with improving insulin resistance with inhibiting the expression and activity of hepatic GSK3β.

关 键 词：黄芪多糖 肝脂肪变 KKAY小鼠 胰岛素抵抗 

分 类 号：R285.5[医药卫生—中药学]