高糖高棕榈酸培养对β细胞脂质含量及脂肪酸转位酶表达的影响  

Effects of supraphysiologic glucose and palmitate on the content of intracellular triglyceride and expression of FAT/CD36 in β-cells

作　　者：李燕[1] 陈璐璐[1] 黎慧清[1] 王咏波[1] 田源[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院内分泌科,武汉430022

出　　处：《中国糖尿病杂志》2008年第4期237-239,共3页Chinese Journal of Diabetes

基　　金：湖北省自然科学基金资助项目(No.2003ABA143)

摘　　要：目的研究高糖高棕榈酸(PA)培养对β细胞脂质含量及脂肪酸转位酶(FAT/CD36)表达的影响。方法NIT-1细胞分别以5mmol/L葡萄糖(NC组)、25mmol/L葡萄糖(HG组)、0.25mmol/LPA+5mmol/L葡萄糖(HP组)及0.25mmol/LPA+25mmol/L葡萄糖(GP组)培养24h后,测定细胞内甘油三酯(TG)含量、胰岛素分泌以及FAT/CD36 mRNA与蛋白的表达。结果(1)与NC组比较,各处理组细胞内TG含量增加,而葡萄糖刺激的胰岛素分泌下降,以GP组变化最明显。(2)在高糖孵育下,HG组和GP组FAT/CD36 mRNA与蛋白表达均较NC组显著增加(P<0.01),但HP和GP组与相应的葡萄糖组比较,FAT/CD36表达无明显变化。结论在高棕榈酸的环境下,高糖可进一步促进β细胞脂质沉积,抑制葡萄糖刺激的胰岛素分泌;其中高糖上调脂肪酸转位酶表达可能是其重要机制之一。Objective To observe the effects of supraphysiologie levels of glucose and palmitate on the intracellular triglyceride （TG） content and FAT/CD36 （FAT： fatty acid translocase） expression in β- cells. Methods NIT-1 cells were exposed to 25 mmol/L glucose （HG group） and 0. 25 mmol/L palmitate with either 5 mmol/L （HP group） or 25 mmol/L glucose （GP group） for 24 h. Control group was provided by cells cultured at 5 mmol/1 glucose. TG content, insulin level and expressions of FAT/CD36 mRNA and protein were detected respectively. Results （1）Cells cultured in elevated glucose and/or palmitate showed an increased TG content and a decreased glucose-stimulated insulin secretion （GSIS） vs; NC group. The most significant changes of TG and GSIS were observed in GP group. （2）FAT/CD36 expression was significantly enhanced in both HG and GP groups vs. NC group（P〈0. 01） ,but palmitate had no effect on the FAT/CD36 expression at either 5 mmol/L or 25 mmol/L glucose. Conclusions In the presence of elevated palmitate levels, the prolonged exposure of β-cells to supraphysiologic concentrations of glucose could further increase TG content and inhibit GSIS, for which the high expression of FAT/CD36 induced by enhanced glucose concentrations may be one of important mechanisms.

关 键 词：Β细胞 脂肪酸转位酶 甘油三酯 胰岛素分泌 

分 类 号：R587.1[医药卫生—内分泌]