亚洲璃眼蜱唾液腺新基因GP15的表达和鉴定  

Expression and Identification of the protein encoded by a novel gene(GP15) from Hyalomma asiaticum tick salivary gland

作　　者：程天印[1] 周金林[1] 周勇志[1] 施启顺[2] 林矫矫[1] 

机构地区：[1]中国农业科学院上海家畜寄生虫病研究所,上海200232 [2]湖南农业大学动物科技学院,湖南长沙410128

出　　处：《中国兽医学报》2008年第2期157-159,共3页Chinese Journal of Veterinary Science

基　　金：科技部基础研究重大项目前期研究专项资助项目(2001CCA00900)

摘　　要：将GP15的阅读框序列插入pET32a(+)制成重组质粒,通过BL21成功表达出相对分子质量为36 000的蛋白。该蛋白可被His.Bind从细菌裂解物纯化出来,也可为抗融合蛋白抗体所识别。表达形式分析显示,融合蛋白以包涵体形式在大肠肝菌内表达。The open reading frame of GP15 was ligated with the linear expression vector pET32a（＋） and the recombinant plasmid was yielded.A novel protein of about 36 000 molecular mass was expressed by the Escherichia coli（BL21 strain）.This protein purified by His·Bind resin,and recognized by the antisera against the fusion protein.The expression type analysis indicates that the fusion protein is expressed in Escherichia coli as inclusion bodies.

关 键 词：亚洲璃眼蜱唾液腺 GP15基因 表达 

分 类 号：S852.746[农业科学—基础兽医学]