RT-PCR检测鹿源致病性大肠杆菌耐药基因blaAmpC的mRNA水平  

Quantitative detection of the expression of drug resistant gene blaAmpC mRNA of enteropathogenic E.coli from sick deer

作　　者：王丽艳[1] 王全凯[2] 王玮[1] 邓旭明[1] 张晶[3] 尹秀玲[4] 郎需龙[1] 柳巨雄[1] 

机构地区：[1]吉林大学畜牧兽医学院 [2]吉林农业大学动物科技学院,吉林长春130118 [3]青岛农业大学,山东青岛266109 [4]河北北方学院,河北张家口075031

出　　处：《中国兽医学报》2008年第2期163-165,共3页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30270999)

摘　　要：应用实时荧光定量RT-PCR对临床分离的10株鹿源大肠杆菌耐药株的blaAmpC基因进行了mRNA水平的定量测定,分析其mRNA转录水平与耐药强度的相关性。结果发现,对β-内酰胺类药物耐药水平高的菌株,其mRNA的表达水平亦高,表明大肠杆菌耐药株blaAmpC基因的mRNA表达水平与其耐药性有高度相关性。Ten Enteropathogenic Escherichia coli clinical isolates were isolated from sick deer and the expression of blaAmpC gene mRNA transcriptionnal level were detected by real-time fluore-scent quantitation RT-PCR,and the relationship between mRNA transcriptional level and drug resistance analyzed.The results showed that the level of resistance and the expression of mRNA was higher.It indicated that the expression level of blaAmpC gene mRNA in resistant E.coli strains was closely related with its drug resistance.

关 键 词：大肠杆菌 实时荧光定量RT-PCR 细菌耐药性 blaAmpC基因 

分 类 号：S852.61[农业科学—基础兽医学] Q783[农业科学—兽医学]