葡萄糖对体外培养的牛脂肪细胞HSL mRNA和ADPN mRNA丰度的影响  

Effect of glucose on abundance of ADPN mRNA and HSL mRNA in vitro culturing bovine adipocytes

作　　者：车英玉[1] 孙国权[2] 张辉[1] 张才[1] 王贵[3] 宋文华[4] 刘国文[1] 王哲[1] 

机构地区：[1]吉林大学畜牧兽医学院动物营养代谢病与中毒病研究室,吉林长春130062 [2]内蒙古民族大学,内蒙古通辽028000 [3]吉林农业大学动物科技学院,吉林长春130118 [4]松原职业技术学院,吉林松原138000

出　　处：《中国兽医学报》2008年第2期200-203,共4页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30571365,30600441);中国博士后科学基金资助项目(2005037638)

摘　　要：在体外牛脂肪细胞培养介质中分别添加0,5,10,15,20,25 mmol/L等不同梯度的葡萄糖,各浓度组均设置3个重复,应用荧光定量PCR技术,观测不同浓度的葡萄糖对体外单层培养的牛脂肪细胞脂联素(ADPN)mRNA与甘油三酯脂肪酶(HSL)mRNA丰度的影响。结果表明,葡萄糖可抑制牛脂肪细胞ADPN mRNA的表达,并促进HSL mRNA的表达。A test was designed to investigate whether the glucose affect the expression of ADPN mRNA and HSL mRNA in culturing adipose cells from newborn Holstein calf.Glucose was added to the media with 0,5,10,15,20,25 mmol/L,respectively.Abundance of ADPN mRNA and HSL mRNA in adipose cells was determined by Fluorescent Quantitative RT-PCR.The results showed that the expression of ADPN mRNA was suppressed and the level of HSL mRNA was elevated in adipose cells treated with glucose.

关 键 词：葡萄糖 牛脂肪细胞 HSL MRNA ADPN MRNA 

分 类 号：S852.21[农业科学—基础兽医学]