大熊猫精浆中PAF-AH和GOT水平与精液质量的关系  

Concentration of platelet-activating factor acetylhydrolase(PAF-AH) and glutamic-oxalacetic transaminase(GOT) in giant panda′s seminal plasma and its relationship with semen quality

作　　者：张明[1] 鲜红[2] 朱庆[1] 侯蓉 郑鸿培[1] 

机构地区：[1]四川农业大学动物科技学院,四川雅安625014 [2]成都市妇产科医院生殖不孕研究所,四川成都610015 [3]成都大熊猫繁育基地濒危野生动物繁殖与保护遗传四川省重点实验室,四川成都610081

出　　处：《中国兽医学报》2008年第2期208-211,215,共5页Chinese Journal of Veterinary Science

基　　金：中国大熊猫繁育研究基金资助项目(2003010)

摘　　要：本研究检测了大熊猫精浆血小板激活因子乙酰水解酶(PAF-AH)和谷草转氨酶(GOT)活性,初步探讨了这些物质与大熊猫精液质量的关系。结果显示,大熊猫精浆存在PAF-AH和GOT,其平均水平分别为(56.76±60.86)mmol.min-1.mL-1和(1 948.9±774.0)IU/L,PAF-AH含量高低与精子活力呈高度负相关(R=-0.73,P<0.001),与精子运动状态呈负相关(R=-0.58,P<0.05);GOT含量高低与精子活力显著负相关(R=-0.66,P<0.01)。这些结果表明,大熊猫精浆PAF-AH和GOT活性之高低可作为评价大熊猫精液质量的参照指标。In the studies,platelet-activating factor acetylhydrolase（PAF-AH） and glutamic-oxalacetic transaminase（GOT） concentration were detected by ELISA and chymic colorimetry respectively.The relationship between PAF-AH,GOT concentration and semen quality were analysed.The results are as follows：PAF-AH and GOT were found in all giant panda seminal,their average concentration were（56.76±60.86） mmol·min^-1·mL^-1 and（1 948.9±774.0） IU/L respectively.Correlation analysis revealed a highly negative correlation between PAF-AH concentration and sperm motility（R=-0.73,P〈0.001）,it also had negative correlation with sperm movement state（R=-0.58,P〈0.05）;seminal GOT concentration just has highly negative correlation with sperm motility（R=-0.66,P〈0.01）.The results indicated that the concentration of PAF-AH and GOT in giant panda seminal plasma maybe a characteristic reference for evaluating the semen quality.

关 键 词：大熊猫 精浆 血小板激活因子乙酰水解酶(PAF-AH) 谷草转氨酶(GOT) 

分 类 号：S814.8[农业科学—畜牧学]