牛卵母细胞的体外成熟  

In vitro maturation of bovine oocytes

作　　者：韦精卫[1] 韦英明[1] 孟凡丽[1] 杨素芳[1] 石德顺[1] 

机构地区：[1]广西大学动物繁殖研究所,广西南宁530005

出　　处：《中国兽医学报》2008年第2期216-219,共4页Chinese Journal of Veterinary Science

基　　金：国家"863"高科技研究发展计划资助项目(2002AA206651);广西大学博士启动基金资助项目(DD150034)

摘　　要：在卵母细胞体外成熟液(含FSH)中分别添加10、30、50μg/L表皮生长因子(EGF),24 h检查成熟率。结果表明,添加10、30μg/L EGF组牛卵母细胞成熟率为79.8%、71.5%与对照组(未添加EGF)成熟率(70.4%)无明显差异(P>0.05),但EGF添加至50μg/L时牛卵母细胞第一极体(PB1)排出率显著提高,达85.4%(P<0.01);在此基础上,比较了成熟液中添加尿促性素(HMG)对牛卵母细胞体外成熟的影响,结果发现在含FSH和EGF的成熟液中添加HMG未能显著提高牛卵母细胞体外成熟效果(P>0.05);最后,比较了HMG对牛卵母细胞体外成熟的效果,结果发现单独添加HMG成熟效果显著高于FSH(P<0.05),表明成熟液中如不含其他生长因子,则单独添加HMG较FSH对牛卵母细胞体外成熟的效果好。Factors affecting maturation of bovine oocytes were studied.The maturation rates of oocytes which cultured at medium with addition of 10,30 μg/L EGF respectively had no significant different with control group（no addition of EGF）,but when the EGF concentration up to 50 μg/L,the rates of Pb1 extruction（85.4%） were significant different from the control group（70.4%）（P〈0.01）;Addition of the HMG to the maturation medium which contain the FSH and EGF couldn＇t promote the rates of nucleus maturation（P〉0.05）;The rates of nucleus maturation of groups which addition HMG alone were higher than addition FSH group（P〈0.05）,indicating that effects of HMG in maturation of bovine oocytes is stronger than FSH.

关 键 词：牛 卵母细胞体外成熟 表皮生长因子 尿促性素 

分 类 号：S814.8[农业科学—畜牧学]