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作 者:张治涛[1] 李玉峰[1] 姜平[1] 王先炜[1] 邓雨修[1]
机构地区:[1]南京农业大学农业部动物疫病诊断与免疫重点开放实验室,江苏南京210095
出 处:《中国兽医学报》2008年第3期227-231,共5页Chinese Journal of Veterinary Science
基 金:教育部重点资助项目(104101);江苏省自然科学基金资助项目(BK2005093);教育部新世纪优秀人才支持计划资助项目(NCET-04-0502)
摘 要:根据GenBank上发表的PCV2 ORF2和PRRSV ORF7序列设计合成引物,建立分别用于检测PCV2和PRRSV的PCR和RT-PCR方法,并对2003年9月至2005年5月采自189个可疑发病猪场的389份病料进行了PCV2和PRRSV的检测。结果显示,来自33个猪场的35份样品为PCV2阳性,猪场PCV2阳性率为17.1%;PCV2与PRRSV混合感染率为5.1%,PCV2阳性猪场中PRRSV感染率为57.6%。流行病学统计结果表明,安徽省猪场PCV2阳性率最高;安徽省和广西省猪场PCV2和PRRSV的混合感染最严重;春夏季节PCV2阳性率明显高于秋冬;消瘦、被毛粗乱是PCV2感染的主要特征;淋巴结水肿或出血,肾脏肿大、出血或呈灰白色以及脾脏病变是PCV2感染的主要病理变化;仔猪7~8周龄发病率最高。According to the published ORF2 sequence of porcine circovirus type 2(PCV2) and ORF7 sequence of porcine reproductive and respiratory disease syndrome virus(PRRSV),the primers were designed and PCR and RT-PCR were set up for the detection of PCV2 and PRRSV,respectively.During Sep.2003 to May.2005,389 samples were collected from 193 suspected farms and detected for PRRSV by RT-PCR and for PCV2 by PCR.As a result,35 clinical samples from 33 farms were positive for PCV2.The positive rate of PCV2 in field was 17.1%,the co-infection rate of PRRSV/PCV2 in farms was 5.1%.The positive rate of PRRSV in PCV2-positive pigs was as high as 57.6%.The statistical results demonstrated that farms in Anhui province got the highest positive rate of PCV2 than other provinces,while farms in Anhui and Guangxi province got the highest rate of co-infection.The results indicated that PCV2 is more prevalent in Spring and Summer than it is in Autumn and Winter.The clinical symptoms of PCV2 associated diseases are mainly characterized by wasting and shag,and the gross lesions are characterized by edema or hemorrhage of lymph node,lung collapse and areas of consolidation in anterior ventral pulmonary areas,and kidney lesions.Furthermore,the study indicated that 7-8 week of age is the most susceptible period during the whole growing stages for pigs.
关 键 词:猪圆环病毒2型 流行病学 混合感染 猪繁殖与呼吸综合征病毒
分 类 号:S852.65[农业科学—基础兽医学]
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