禽网状内皮组织增生病病毒和呼肠孤病毒感染SPF鸡对IFN-γ产生的影响  

Effects of reticuloendotheliosis virus and reovirus infection on IFN-gamma production in SPF chickens

作　　者：郑玉姝[1] 赵宏坤[1] 崔治中[1] 赵朴[2] 李宏梅[1] 刘翠艳[1] 李培庆[2] 

机构地区：[1]山东农业大学动物科技学院,山东泰安271018 [2]河南科技学院动物科技学院,河南新乡453003

出　　处：《中国兽医学报》2008年第3期232-234,238,共4页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金重点资助项目(30330450)

摘　　要：1日龄SPF鸡分别接种禽网状内皮组织增生病病毒(REV)、禽呼肠孤病毒(ARV)和REV+ARV,于感染后3、5、46 d采集脾细胞做脾淋巴细胞培养并采用双抗体夹心ELISA法检测IFN-γ水平。结果表明,与对照相比,鸡感染REV后脾淋巴细胞IFN-γ的分泌水平显著升高。感染后5 d,REV单独感染引起IFN-γ8倍的增长,达到峰值,然后逐渐下降。ARV单独感染引起IFN-γ2倍的增长。REV和ARV共感染鸡脾淋巴细胞IFN-γ的分泌量增长了3倍。至感染后46 d,共感染和REV单独感染的IFN-γ水平与对照组相比仍然差异显著,ARV单独感染与对照组相比差异不再显著。Specific-pathogen-free（SPF） chickens were infected with REV,ARV or REV＋ARV at 1 day of age,spleen was collected for preparation of lymphocytes and examine from 3,5 and 46 days post infection（dpi）.Levels of IFN-γ were measured by double mAb sandwich ELISA.The results showed that IFN-γ levels in spleen lymphocytes significantly increased in chickens infected with REV alone as compared with the control.At 5 dpi,REV alone caused a 8-fold increase and the IFN-γ levels peaked,and then gradually decreased.ARV alone caused a 2-fold increase.In chickens coinfected with REV and ARV,IFN-γ ELISA demonstrated a 3-fold increase in IFNγ levels.At 46 dpi,a significant increase was found in IFN-γ levels in coinfected chickens and chickens with REV alone as compared with age-matched control,but an insignificant increase in chickens with ARV alone as compared with control.

关 键 词：IFN-Γ 禽呼肠孤病毒 禽网状内皮组织增生病病毒 SPF鸡 

分 类 号：S852.65[农业科学—基础兽医学] S852.4[农业科学—兽医学]