PHS对快大型黄羽肉鸡线粒体NO代谢和Na^＋-K^＋-ATP酶活力的影响  

Metabolism of NO and Na^+-K^+-ATPase of mitochondria during controlling PHS in fast-growing yellow broilers

作　　者：臧莹安[1] 丁发源[2] 王小龙[3] 向瑞平[4] 

机构地区：[1]仲恺农业技术学院,广东广州510225 [2]广东省农业科学院畜牧研究所,广东广州510640 [3]南京农业大学动物医学院,江苏南京210095 [4]郑州牧业工程高等专科学校,河南郑州450045

出　　处：《中国兽医学报》2008年第3期306-309,共4页Chinese Journal of Veterinary Science

基　　金：中国博士后科学基金资助项目(2003033411);仲恺农业技术学院引进人才基金资助项目(G2360255)

摘　　要：采取分组对比的方法分别检测了对照组、PHS发病组(低温和日粮中添加1.5 mg/kg T3)、防治组(日粮中添加500 mg/kg Vc)快大型黄羽肉鸡肝组织、肠黏膜、心肌线粒体中的NO含量、NOS的活性和Na+-K+-ATP酶活力。结果显示,发生PHS的黄羽肉鸡,其肝脏、心肌和肠黏膜线粒体NO活力呈现先显著上升(至PHS处理后1、2周)后显著下降(3~5周)的变化趋势(P<0.O5)。线粒体NOS活力和Na+-K+-ATP酶活力的变化趋势则与NO一致。提示:缺氧的早期,NOS活性升高从而使NO水平升高,NO水平升高舒张血管,缓解肺动脉高压,是机体的一种应激反应。随着缺氧时间的延续,机体NOS和NO合成相对不足,相应器官的功能受到损害,肺血管舒缩失衡出现肺动脉高压,进一步发生腹水。而Na+-K+-ATP酶活力的改变则使能量代谢发生障碍,ATP生成减少,引起了线粒体的损伤,进一步诱导了肉鸡PHS;日粮中添加500 mg/kg Vc对此过程具有明显的颉颃作用,对机体起到保护作用。A total of 120 broilers were divided randomly into three groups that were respectively treated as healthy group,the pulmonary hypertension syndrome（PHS） group and the vitamin treatment group.The liver,heart and intestinal mucous membrane were taken from 6 killed birds per group,and its mitochondria were determined for the evaluations of the concentrations of nitrix oxide（NO）,nitric oxide synthase（NOS） and Na＋-K＋-ATPase.The results indicated that：From 1 week to 2 weeks,the NO,NOS and Na＋-K＋-ATPase level of the mitochondria in liver,heart and intestinal mucous membrane in broilers increased significantly（P〈0.05）.But from 3 weeks to 5 weeks,decreased significantly（P〈0.05）.After the treatment of vitamin C,all these guideline changed following opposite way.It might be concluded that during the early period of the oxygen deficit spending,the activeness of NOS elevates,thus causes the NO level elevate,this diastoles the vessel,alleviates the high pressure of pulmonary artery,is one kind of body stress reaction.But if the oxygen deficiting continuring,body can not react efficiently to alleviate this,thus the adaption comes into being,the synthesize of NOS and NO relatively insufficient.The function of corresponding organ is damaged,the unbalancedly stretch and constringency of the lung blood vessel results the pulmonary artery high pressure,further lead to the pulmonary hypertension syndrome（PHS）,the changes of Na^＋-K^＋-ATPase result in the disfunction of the energy metabolism,decreased the production of ATP energy.Some medicines may play an important role in the objection of PHS in yellow broiler chickens and the protection of yellow broilers.

关 键 词：黄羽肉鸡 PHS 线粒体 自由基 NO NOS NA^+-K^+-ATP酶 

分 类 号：S856.2[农业科学—临床兽医学] S852.33[农业科学—兽医学]