调节胚胎代谢对蛋鸡激素、抗氧化及产蛋性能的影响  

Effects of regulating embryonic metabolism on hormone level and antioxidation performance and laying performance in laying hens

作　　者：孙镇平[1] 华棣[1] 秦玉玲[1] 吕文亭[1] 王怀蓬[1] 刘艳玲[1] 

机构地区：[1]扬州大学兽医学院,江苏扬州225009

出　　处：《中国兽医学报》2008年第3期319-324,共6页Chinese Journal of Veterinary Science

摘　　要：应用VE、VC及普鲁卡因作为胚胎调节剂对蛋鸡胚胎生长发育进行调控。225枚种蛋随机分为3组,即对照组,不采取任何处理;试验1组,采用（78%VE14μL＋25%VC20μL）/枚;试验2组,采用（78%VE14μL＋25%VC20μL＋2%普鲁卡因56μL）/枚;于入孵前,距离种蛋钝端1/4近气室处,经酒精消毒,将调节剂液注射到种蛋胚盘附近,探讨其对蛋鸡内分泌水平、抗氧化性能、免疫性能及生产性能的影响。结果表明：与对照组相比,15周龄蛋鸡激素水平,1组卵泡刺激素（FSH）、促黄体生成素（LH）水平均极显著提高（P〈0.01）;2组甲状腺素（T4）水平显著提高（P〈0.01）。39周龄时,1、2组T4水平显著升高（P〈0.05或P〈0.01）,2组孕酮（P）、胰高血糖素水平显著提高（P〈0.05）;抗氧化性能,试验组血清和肝脏中丙二醛（MDA）含量均不同程度地降低,其中2组血清MDA显著降低（P〈0.05）,其余指标差异不显著;脂类代谢方面,试验2组蛋黄胆固醇降低16.58%（P〈0.05）,1组蛋黄粗脂肪降低13.51%（P〈0.05）。对新城疫（ND）疫苗免疫的影响,试验组在注射ND疫苗后7、14、28 d抗体效价不同程度地提高,其中1组在14、28 d时抗体效价差异极显著（P〈0.01）。此外,0 d,1组的抗体效价比2组提高58.35%,差异显著（P〈0.05）;28 d,1组的抗体效价比试验2组提高29.03%（P〈0.01）;其他时间点2个试验组间差异不显著。在22~39周龄,试验1、2组平均蛋重和总产蛋量都不同程度地提高,1组尤为显著（P〈0.01或P〈0.05）,而试验组料蛋比均显著性降低（P〈0.05）。因此,通过调节胚胎代谢能明显改善出壳后蛋鸡的内分泌水平及抗氧化性能,提高产蛋性能。Vitamin E,vitamin C and procaine were applicated to regulate embryonic the growth and development of laying hens in this study.225 eggs were divided at random into three groups,the control group did not take any treatment,the test group 1 was injected（78%VE 14 μL＋25%VC 20 μL）/egg,the test group 2 was injected（78%VE 14 μL＋25%VC 20 μL＋2% procaine 56 μL）/egg.Before incubating,eggs blunt end 1 / 4 near the gas chamber,regulating fluid was injected into eggs.That changes of endocrine metabolism,antioxidant property,immune property and production performance were observed in laying hens.The results showed that,compared with control group,at 15 weeks of age,serum follicle-stimulating hormone（FSH） and luteinizing hormone（LH） concentration in treated group 1 significantly increased（P〈0.01）,serum thyroid hormone（T4） concentration in treated group 2 significantly increased（P〈0.05）.At 39 weeks of age,serum T4 concentration in treated groups increased differently,and serum progesterone（P）,LH and Glucagon（Glu） concentration significantly increased（P〈0.05）.Serum and liver MDA level in treated groups decreased differently,especially serum MDA in treated group 2 significantly decreased（P〈0.05）,but had no significant effect on other indices measured.Yolk cholesterol content of treated group 2 significantly decreased by 16.58%（P〈0.05） and yolk adipose content of treated group 1 significantly decreased by 13.51%（P〈0.05）.Antibody titer on 7,14,28 d after injecting Newcastle disease（ND） bacterin increased differently in treated groups,especially those of 7 d and 14 d in treated group 1 significantly increased（P〈0.01）.During 22-39 weeks of old,average egg weigh and total egg production of treated groups increased,especially treated group 1 significantly increased by 7.93%（P〈0.01） and 6.00%（P〈0.05）,respectively.Feed/egg ratio significantly decreased in treated groups（P〈0.05）.Eggshell thickness in treated group 1 increased by 8.82%（P�

关 键 词：蛋鸡 胚胎代谢调节 内分泌水平 抗氧化性能 产蛋性能 

分 类 号：S831.5[农业科学—畜牧学] S814.8[农业科学—畜牧兽医]