三江白猪心脏脂肪酸结合蛋白(H-FABP)基因多态性及多态片段的序列分析  

Polymorphism and sequence analysis of polymorphic fragments of H-FABP gene of Sanjiang white pigs

作　　者：黄大鹏[1] 黄玉兰[1] 陈建华[1] 

机构地区：[1]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319

出　　处：《中国兽医学报》2008年第3期336-338,342,共4页Chinese Journal of Veterinary Science

基　　金：大庆市科技局2004年科技攻关资助项目(SGG04-076)

摘　　要：利用PCR-RFLP方法测定80头三江白猪H-FABP基因的多态性并对其多态性片段进行了序列分析。结果表明,HinfⅠ-RFLP和MspⅠ-RFLP位点上没有表现多态性,基因型分别为HH和AA型;只存在HaeⅢ-RFLP和HinfⅠ*-RFLP位点多态性,基因型分别为DD、Dd、dd和Bb、bb型,等位基因b的频率为0.95,等位基因d的频率为0.75。在HinfⅠ*-RFLP位点上,PIC为低度多态(PIC<0.25);HaeⅢ-RFLP位点上PIC为中度多态(0.25<PIC<0.5)。对此HaeⅢ-RFLP多态片段进行了克隆测序分析,HaeⅢ-RFLP变异的酶切位点在1 811位,发生了C到G的突变。测序结果与GenBank所公布的序列X98558和Y16180分别有99.4%和97.4%的同源性。The genotypes of 80 Sanjiang white pigs for H-FABP locus were detected by PCRRFLP method.The results revealed that none of polymorphism is determined in Hinf Ⅰ-RFLP and Msp Ⅰ-RFLP,their genotype is HH and AA respectively.Only Hae Ⅲ-RFLP and HinfⅠ＊-RFLP is verified,the genotypes is DD,Dd,dd and Bb,bb.The frequencies of b allele were 0.95;The frequencies of d were 0.70.For Hinf Ⅰ-RFLP,Sanjiang white pig had low polymorphism（PIC〈0.25）;For Hae Ⅲ-RFLP,Sanjiang white pig had low intermediate polymorphism（0.25）; For Hae IH-RFLP, Sanjiang white pig had low intermediate polymorphism （0.25 〈 PIC 〈 0.5）. The Hae m-RFLP polymorphlc fragment were cloned and sequenced. The results revealed a single nueleotide substitution of C→G at position 1 811 for Hae Ⅲ-RFLP. Identified 816 bp and 693 bp segment of clone by sequence, the results demonstrated that nucleotide homology with published H-FABP genes on GenBank Y16180 and X98558 were 99.4% and 97.4% respectively.

关 键 词：三江白猪 H-FABP基因 RFLP 序列分析 

分 类 号：S813[农业科学—畜牧学] Q78[农业科学—畜牧兽医]