尿中锰的火焰原子吸收分光光度直接测定法  

Direct Determination of Manganese in Urine by Flame Atom Absorption Spectrophotometry

作　　者：朱正强[1] 刘毅[1] 

机构地区：[1]四川省雅安市疾病预防控制中心检验科,四川雅安625000

出　　处：《环境与健康杂志》2008年第2期160-161,共2页Journal of Environment and Health

摘　　要：目的建立尿中锰的火焰原子吸收分光光度直接测定法。方法采用火焰原子吸收分光光度法直接测定尿锰,并与湿式消解法进行比较。结果方法的线性范围为0.01～0.80μg/ml,线性方程为y=0.00187+0.7853x,r=0.9997,最低检测浓度为0.01μg/ml,检出范围为0.01～0.80μg/ml,RSD为0.056%。加标0.02和0.04μg/ml的平均回收率为95.1%。该法与湿式消解法测定尿锰含量结果比较,测定结果的差异无统计学意义(P>0.05)。结论尿中锰的火焰原子吸收分光光度直接测定法简便,快速,准确,可用于日常监测。Objective To establish the methods for direct determination of manganese in the urine by flame atom absorption spectrophotometry （AAS）. Methods Manganese in the urinary samples were determined directly and the result was compared with that by acid digestion. Results The results showed a good linearity, from 0.01 to 0.80 μg/ml, r=0.9997, y=0.001 87＋0.785 3 x. For the same urinary sample, the average urinary manganese content was 0.027 μg/ml, standard deviation was 0.000 015 μg/ml, RSD=0.056%. The average recovery rate was 95.1%. No significant difference was seen compared with the acid digestion. Conclusion The direct determination of urinary manganese by AAS is simple, rapid, accurate and feasible.

关 键 词：分光光度法 原子吸收 尿锰 测定 

分 类 号：O657.3[理学—分析化学]