氯唑西林对阴沟杆菌β-内酰胺酶的抑制及其与头孢他啶的协同抗菌作用  

Inhibitory effect of cloxacillin on β lactamases from ceftazidime resistant Enterobacter cloacae and its synergistic antibacterial action combined with ceftazidime

作　　者：凌保东[1] 雷军[1] 

机构地区：[1]川北医学院

出　　处：《中国抗生素杂志》1997年第5期364-367,共4页Chinese Journal of Antibiotics

摘　　要：采用紫外分光光度法比较了氯唑西林和舒巴坦对头孢他啶耐药阴沟杆菌β－内酰胺酶的抑制作用，并对氯唑西林与头孢他啶协同抗耐药菌的作用进行了探讨。结果表明：细菌对头孢他啶耐药后，对其它β－内酰胺类抗生素也呈现交叉耐药；以头孢噻啶或头孢孟多为底物时，氯唑西林（１～５μｇ／ｍｌ）对头孢他啶耐药菌株的β－内酰胺酶有明显抑制作用；氯唑西林与头孢他啶联合对耐药菌呈协同抗菌作用；未见两药间的拮抗效应。The inhibitory action of cloxacillin (CLX) and sulbactam (SBT) on β lactamases from ceftazidime (CTZ) resistant bacteria were comparatively determined by ultraviolet spectrophotometry, and the combination of CLX and CTZ against CTZ resistant bacteria was studied. The results showed that: when cefaloridine (CER) or cefamandole (CMD) (0.2mmol/ml) used as the substrate, CLX (1￣5μg/ml) exhibited stronger inhibitory action on β lactamases from CTZ resistant Enterobacter cloacae than SBT ( P < 0.05 ); as soon as occured resistance of bacteria to CTZ, the bacteria were resistant to β lactam antibiotics, including the third generation cefalosporins; the combination of CLX and CTZ in vitro showed that a remarkably synergistic activity against CTZ resistant Enterob acter cloacae .

关 键 词：氯唑西林 舒巴坦 头孢他啶 Β-内酰胺酶 耐药性 

分 类 号：R978.1[医药卫生—药品] R969.2[医药卫生—药学]