早孕小鼠子宫内膜mTOR基因表达增高  

Expression Increasing of mTOR Gene in Endometrium of Early Pregnant Mouse

作　　者：曾兰[1] 何俊琳[2] 丁裕斌[2] 陈雪梅[2] 王应雄[2] 刘学庆[1] 

机构地区：[1]重庆医科大学公共卫生学院生殖生物学研究室,重庆400016 [2]重庆医科大学公共卫生学院遗传优生教研室,重庆400016

出　　处：《细胞生物学杂志》2008年第2期211-216,共6页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(No.30500054);重庆市人口与计划生育委员会自然科学基金(06-01)资助项目~~

摘　　要：利用实时荧光定量PCR、原位杂交法、免疫组化(SP法)和Western印迹法分别检测未孕、假孕及孕d3、d4、d5、d6、d7小鼠子宫内膜哺乳动物雷帕霉素靶蛋白(mammaliantargetofrapamycin,mTOR)mRNA和蛋白质的表达,研究mTOR基因在早孕小鼠子宫内膜的表达规律。结果显示妊娠子宫内膜组织mTOR的表达较未妊娠的子宫内膜组织显著增加(P<0.05);着床窗期表达最高(d4,d5),且与其他妊娠各期比较差异有统计学意义(P<0.05);原位杂交和免疫组化分析显示mTORmRNA和蛋白质表达主要在子宫内膜基质细胞,与上皮细胞各组比较差异有统计学意义(P<0.05)。研究表明mTOR在子宫内膜基质细胞的规律性表达所调控的细胞生长、增殖和分化可能是胚胎正常着床的分子机制之一。To explore the expression rule of mammalian target of rapamycin （mTOR） gene in endometria of early pregnant mouse, real-time fluorescence quantitative PCR （RT FQ-PCR）, in situ hybridization, immunohistochemistry and Western blotting were used to detect the mTOR mRNA and protein in the mouse endometria from unpregnant, pseudopregnant and pregnant d3, d4, d5, d6 and d7. Results showed that the mTOR expression in pregnant groups was higher than that of non-pregnant group （P〈0.05）, and the mTOR expression increased significantly in the implantation window （P〈0.05）. By in situ hybridization and immunohistochemistry, mTOR expression of stroma cell in endometria was higher than that of uterine epithelium cell （P〈0.05）. The above results indicated that the expression rule of the mTOR in endometrial stromal cell may be one of the implantation mechanisms by regulating cell growth, proliferation and differentiation.

关 键 词：哺乳动物雷帕霉素靶蛋白 胚泡着床 子宫内膜 实时荧光定量PCR 原位杂交 

分 类 号：Q344[生物学—遗传学]