人骨髓基质细胞输注裸鼠后的体内分布  

Distribution of Human Bone Marrow-derived Stromal Cells Following Intravenous Injection into Nude Mice

作　　者：邹维艳[1,3] 邝晓聪[1] 韦瑛[1] 朱名毅[1] 黄英华[1] 温冠媚[2] 

机构地区：[1]广西医科大学病理生理学教研室,南宁530021 [2]广州医学院病理生理学教研室,广州510182 [3]蚌埠医学院组织胚胎学教研室,蚌埠233003

出　　处：《细胞生物学杂志》2008年第2期217-221,共5页Chinese Journal of Cell Biology

基　　金：广西自然科学基金(青0447026);广西留学回国人员科学基金(桂科回0731016)资助~~

摘　　要：采用密度梯度离心和差速贴壁法分离培养人骨髓基质细胞。对细胞进行形态观察、表型分析、细胞生长曲线和成骨分化潜能测定。然后将第4代细胞通过尾静脉输注到未经任何处理的裸鼠体内,移植1周和4周后,利用RT-PCR检测人Alu基因在裸鼠不同脏器组织中的表达以判断人源性供体细胞的植入情况。结果表明培养的人骨髓基质细胞为CD34-CD45-成纤维样细胞,并具有向成骨分化的能力,但第1至第5代人骨髓基质细胞增殖速度无显著性差异(P>0.05)。经尾静脉输注后,人源性供体细胞可在肺、肝、脾、肾、肌肉、心、肠、脑等多种脏器或组织中均有不同程度的分布。human bone marrow-derived stromal cells （hBMSCs） were isolated by gradient centrifugation and were ex vivo-expanded using adhesion culture method. Morphology, phenotypes, cell growth dynamics, and osteogenic differentiation of hBMSCs were analyzed prior to intravenous injection into unconditioned nude mice. Culture-expanded hBMSCs were subsequently infused into the unconditioned recipients via tail vein injection. The donor-derived engraftment was qualified by reverse transcription polymerase chain reaction （RT-PCR） targeting human Alu gene at one week and four weeks postsystemic injection. The current results demonstrated that ex vivo- expanded hBMSCs phenotypically and morphologically appeared as CD34^–CD45^– spindle-shaped fibroblastic cells with the ability to give rise to osteogenic lineage in vitro. However analysis of growth dynamic of hBMSCs （P1–P5） showed no significant differences （P〉0.05）. Infused cells were present in multiple organs or tissues recovered from the recipients including lungs, liver, spleen, kidneys, skeletal muscle, heart, small intestines, and brain at 7 days and 4 weeks after cell transplantation. These data indicate that ex vivo-expanded hBMSCs distribute broadly following systemic infusion.

关 键 词：骨髓 基质细胞 移植 分布 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]