血管平滑肌细胞在自发性高血压大鼠颈动脉重构中的作用及替米沙坦的干预研究  

The Role of Vascular Smooth Muscle Cells in Carotid Arteries Remodeling of Spontaneously Hypertensive Rats and the Intervention Study of Telmisartan

作　　者：陈宏[1,2] 洪华山[1] 刘永平[3] 马建芳[3] 江琼[1] 

机构地区：[1]福建医科大学附属协和医院心血管内科 [2]南京军区福州总医院476医院,福州350002 [3]南京军区福州总医院476医院

出　　处：《细胞生物学杂志》2008年第2期257-264,共8页Chinese Journal of Cell Biology

基　　金：福建医科大学附属协和医院重点学科基金资助项目~~

摘　　要：观察血管平滑肌细胞(VSMCs)在自发性高血压大鼠(SHR)颈动脉重构中的作用及替米沙坦的干预效果。将30只12周龄的SHR随机分为高血压组(SHR)、替米沙坦高剂量组(TelH)、替米沙坦低剂量组(TelL),另设同性别、周龄的WKY大鼠为对照组(n=10),干预18周。观察各组大鼠收缩压(SBP)、颈动脉中膜厚度(MT)、中膜横截面积(MCSA)、中膜细胞平均核面积、颈动脉VSMCs增殖指数(PI)及凋亡指数(AI)等的变化。结果显示:①两周后TelH组SBP明显低于SHR组(P<0.01),其降压作用持续至实验结束,而TelL组SBP与SHR组无显著性差异(P>0.05);②SHR组的MT、MCSA分别明显高于WKY组(P<0.01),TelH组的MT、MCSA分别明显低于SHR组(P<0.01),TelL组的MT明显低于SHR组(P<0.05);③SHR组中膜VSMCs平均核面积明显大于WKY组(P<0.01),而TelH、TelL组分别小于SHR组(P<0.05);④各组颈动脉中膜VSMCs的PI均无明显差异(P>0.05);SHR组颈动脉中膜VSMCs的AI明显低于WKY组(P<0.01),而TelH、TelL组明显高于SHR组(P<0.01);SHR组颈动脉中膜VSMCs的PI/AI明显高于WKY组(P<0.01),而TelH、TelL组明显低于SHR组(P<0.01);⑤颈动脉中膜VSMCs的AI与中膜MCSA呈显著负相关(r=-0.871,P<0.01)。说明VSMCs的肥大和增殖/凋亡失衡可能在SHR颈动脉重构中起重要作用,替米沙坦除降压作用外,能通过减轻VSMCs肥大,增加VSMCs凋亡,使增殖/凋亡趋于平衡而减轻其重构。To investigate the role of vascular smooth muscle cells （VSMCs） and the effects of telmisartan on carotid arteries remodeling in spontaneously hypertensive rats （SHR）. 12 week-old-male SHRs were randomized to 3 groups （n=10 for each） , including untreated SHR group, telmisartan high dose （TelH） group and telmisartan low dose （TelL） group. Age and sex matched Wistar Kyoto rats were served as control group （WKY）. Systolic blood pressure （SBP） of rats, media thickness （MT）, medial cross-sectional area （MSCA）, mean nuclear area, VSMCs proliferating index （PI） and apoptosis index （AI） of carotid arterial were studied. The results showed that： ① The SBP level in TelH group was significantly decreased when compared to that in SHR group （P〈0.01）. Moreover, the lower SBP in TelH group was kept until to the end of experiment. However, there was not different between SHR group and TelL group （P〉0.05）. ② The MT, MCSA in SHR group were higher than those in WKY group respectively （P〈0.01 for each）. The MT, MCSA in TelH group were lower than those in SHR group respectively （P〈0.01 for each）. The MT was reduced in TelL group when compared with SHR group （P〈0.05 ）. ③ The mean nuclear area of carotid arterial media of both TelH and TelL group was significantly lower than that in SHR group respectively （P〈0.05 for each）. ④ PI was not obviously difference in each group （P〉0.05）. AI in SHR group was lower than that in WKY group （P〈0.01）. AI in TelH, TelL group was higher than that in SHR group respectively （P〈0.01）. PI/AI in SHR group was higher than that in WKY group （P〈0.01）. PI/AI in TelH, TelL group was lower than that in SHR group （P〈0.01）. ⑤ AI in carotid arterial media was significantly negative correlated with medial cross-sectional area （MSCA） （r=–0.871, P〈0.01）. To sum up both hypertrophia and proliferation-apoptosis ratio disequilibrium of VSMCs may play an important role in the pathogenesis

关 键 词：自发性高血压大鼠 颈动脉重构 血管平滑肌细胞 替米沙坦 

分 类 号：R544.1[医药卫生—心血管疾病]