RNAi沉默STAT3对人胰腺癌细胞侵袭能力的影响  

Effect of RNAi-mediated STAT3 gene silencing on the invasiveness of human pancreatic cancer cells

作　　者：黄陈[1] 裘正军[1] 江弢[1] 朱麟[1] 张放[1] 黄克俭[1] 曹俊[1] 

机构地区：[1]上海交通大学附属第一人民医院普外科,200080

出　　处：《中华普通外科杂志》2008年第4期292-295,共4页Chinese Journal of General Surgery

基　　金：上海市教育委员会科研项目（06BZ067）

摘　　要：目的探讨RNA干扰（RNA interference，RNAi）沉默信号转导与转录激活因子-3（signal transduction and activators of transcription，STAT3）对人胰腺癌细胞株SW1990侵袭能力的影响及其机制。方法构建STAT3短发卡RNA（short hairpin RNA，shRNA）表达载体，稳定转染SW1990细胞，RT-PCR和Western blot观察STAT3 mRNA和蛋白表达的改变，体外侵袭实验检测细胞侵袭能力的变化，RT-PCR和Western blot分别检测基质金属蛋白酶-2（matrix metalloproteinase-2，MMP-2）和血管内皮生长因子（vascular endothelial growth factor，VEGF）mRNA和蛋白表达的改变。结果STAT3 shRNA表达载体稳定转染SW1990细胞可显著抑制STAT3 mRNA和蛋白表达；体外侵袭实验显示RNAi沉默STAT3后，SW1990细胞侵袭能力明显下降；RT-PCR和Western blot显示RNAi沉默STAT3后，SW1990细胞中MMP-2和VEGF的mRNA和蛋白表达明显减低。结论STAT3 shRNA表达载体能有效抑制STAT3的表达，并通过下调MMP-2和VEGF表达，抑制胰腺癌细胞体外侵袭能力。RNAi沉默STAT3可能为预防和治疗胰腺癌的侵袭转移提供一种新的策略。Objective To investigate the effect of RNAi-mediated STAT3 gene silencing on the invasiveness of human pancreatic cancer cells. Methods STAT3 shRNA expression vector was stably transfected to SW1990 cells. STAT3 mRNA and protein expression were examined using reverse transcription polymerase chain reaction（RT-PCR） and Western blot, respectively. The invasion ability of SW1990 cells was determined by cell invasion assay in vitro. RT-PCR and Western blot were performed to detect the mRNA and protein expression of the MMP-2 and VEGF, respectively. Results mRNA and protein expression of STAT3 were inhibited significantly by stable transfection of STAT3 shRNA expressing vectors. STAT3 silence with RNAi significantly inhibited the invasion ability of SW1990 cells decreasing protein and mRNA expression of MMP-2 and VEGF in SW1990 cells. Conclusion STAT3 silence with RNAi significantly inhibits the invasion ability of pancreatic cancer cells through down-regulating MMP-2 and VEGF.

关 键 词：胰腺肿瘤 RNA干扰 信号传导及转录 侵袭 转移 

分 类 号：R686[医药卫生—骨科学]