牛初乳短链IGF-Ⅰ对STZ糖尿病大鼠心肌细胞Ca^(2+)的影响  

THE EFFECT OF TRUNCATED IGF-Ⅰ FROM BOVINE COLOSTRUM ON MYOCARDIAL CALCIUM METABOLISM IN STZ-DIABETIC RATS

作　　者：段文澜[1] 俞茂华[1] 沈隽 孙侃[1] 方京冲[1] 朱禧星[1] 颜怡谦[2] 

机构地区：[1]上海医科大学华山医院糖尿病研究所,200040 [2]中国科学院上海生理研究所,200032

出　　处：《上海医科大学学报》1997年第6期403-406,共4页Journal of Fudan University(Medical Science)

摘　　要：为了解牛初乳短键类胰岛素样生长因子-I（IGF－I）对糖尿病大鼠心肌细胞Ca2+的影响，我们用牛初乳短键IGF－I治疗涟豚佐菌素（STZ）糖尿病大鼠。利用原子光谱吸收法测定发现STZ糖尿病大鼠心肌细胞Ca2+的含量明显高于正常对照组[分别为（54．5±9．6）及（24．4±80）μg/g心肌]；牛初乳短键IGF－I口服（15μg／kg体重）或腹腔注射（250ng/kg体重）治疗5周后的糖尿病大鼠血清IGF一I浓度有显著增加，心肌细胞Ca2+含量显著降低［分别为（37．3±14．5）及（27．5±9．7）μg／g心肌]，达正常水平。提示牛初乳短链IGF－I能逆转糖尿病时心肌细胞Ca2+超载现象，为糖尿病心肌病变提供了一种可能的预防措施。PURPOSE In order to investigate the effect of truncated insulin growth factor - I (IGF - I ) from bovine colostrum on myocardial calcium content in STZ - diabetic rats.METHODS Diabetic rats were established by STZ; myocardial calcium content with aton spectrum absorption method; blood glucose with glucose oxidase method; serum insulin, C - peptide and IGF-I with radioimmunoassay. RESULTS Serum IGF - I was greatly increased in truncated IGF - I treated STZ - diabetic rats.Myocardial calcium content was significantly increased in STZ - diabetic rats [ (54. 5 ± 9. 6)μg/g wet wt] compared with that in normal control group [ (24. 4 ± 8. O)μg/g wet wt] (P< O. 0O1 ) and was reduced to normal level in STZ - diabetic rats treated with truncated IGF - I from dorine colostrum both orally 15 μg/kg b. wt. /day and intraperitoneally 250 ng/Kg b- wt. /day for 5 weeks [ (37. 3 ± 14. 5)μg/g wet wt and (27. 5 ± 9. 7)μg/gwet Wt, respecthively].CONCLUSTNS This study suggested that the truncated IGF - I extracted from bovine colostrum can reverse the myocardial Ca2+ overload in STZ- diabetic rats and may become a promising candidate for the prevention of diabetic cardiomyopathy.

关 键 词：IGF-Ⅰ 链脲佐菌素 糖尿病 心肌细胞 钙离子 

分 类 号：R587.1[医药卫生—内分泌]