PCR对可疑慢性淋病病人淋病奈瑟菌的检测  

DETECTION OF NEISSERIA GONORRHEA BY PCR IN THE SUSPICIOUS PATIENTS WITH CHRONIC GONORRHEA

作　　者：臧云娟[1] 王英才[1] 刘桂珍[1] 薛静波[1] 王瑞东[1] 

机构地区：[1]青岛医学院

出　　处：《青岛医学院学报》1997年第4期336-337,共2页Acta Academiae Medicinae Qingdao Universitatis

摘　　要：①目的明确临床上可疑慢性淋病病人的病原学诊断。②方法用聚合酶链反应（ＰＣＲ）对７６例可疑慢性淋病病人的尿道分泌物进行了淋病奈瑟菌检测，并与传统的细菌分离培养法的敏感性进行了比较。③结果聚合酶链反应测出阳性标本２２份（２８．９％），分离培养法测出阳性标本８份（１０．５％）。两种方法敏感性比较，差异有显著性（χ２＝１２．０７，Ｐ＜０．０１）。④结论与细菌培养法相比ＰＣＲ敏感而快速，是明确慢性淋病病原学诊断和指导治疗的好方法。Objective To make clear the pathogenic diagnosis for the patients suspected of chronic gonorrhea. MethodsSeventysix urethral specimens taken from patients suspected of chronic gonorrhea were examined by PCR, conventional culture medium for neisseria gonorrhea were used as control. Results Twentytwo specimens were positive by PCR (28.9%), and 8 specimens were positive by conventional culture medium (10.5%). PCR was more sensitive than conventional culture medium (χ2=1207,P<001). Conclusion PCR is a sensitive and quick method for diagnosing chronic gonorrhea and helpful in guiding the treatment.

关 键 词：淋病奈瑟菌 慢性 淋病 聚合酶链反应 

分 类 号：R759.204[医药卫生—皮肤病学与性病学] R446.9[医药卫生—临床医学]