侧脑室注射β-内啡肽及其抗血清对大鼠血浆唾液酸水平的影响  

THE EFFECT OF LATERAL VENTRICULAR INJECTION OF BETAENDORPHIN AND ITS ANTISERUM ON PLASMA SIALIC ACID LEVEL IN RAT

作　　者：梁福波[1,2,3] 孙瑞卿[1,2,3] 陈蕾[1,2,3] 陈文芳 陈家津[1,2,3] 

机构地区：[1]青岛市市立医院病理科 [2]青岛医学院病理生理学教研室 [3]青岛医学院生理学教研室

出　　处：《青岛医学院学报》1997年第4期342-343,共2页Acta Academiae Medicinae Qingdao Universitatis

摘　　要：①目的探讨β－内啡肽（β－ＥＮＤ）对大鼠血浆唾液酸（ＳＡ）水平的影响。②方法在Ｗｉｓｔａｒ大鼠侧脑室分别注射浓度为２５，５０和１００μｍｏｌ／Ｌ的β－ＥＮＤ（生理盐水对照）和抗β－ＥＮＤ血清（正常兔血清对照），注射前及注射后５，１５，２５和３５ｍｉｎ分别取血，紫外分光光度仪测ＳＡ含量。③结果注射β－ＥＮＤ后大鼠血浆ＳＡ水平较注射前均降低，并且随β－ＥＮＤ浓度的增加，ＳＡ水平降低所需的时间逐渐缩短；注射抗β－ＥＮＤ血清后大鼠血浆ＳＡ水平较注射前升高。④结论外源性及内源性的β－ＥＮＤ均参与了降低大鼠血浆ＳＡ水平的调节过程，并且呈一定的量效依赖关系。Objective To investigate the effect of betaendorphin(βEND) on plasma sialic acid(SA) level of rats. Methods βEND with concentrations of 25,50,100μmol/L(normal saline as control) and its antiserum(normal rabbit serum as control) were injected into the lateral ventricle of Wistar rats respectively. Ultraviolet spectrophotometer was used to determine the plasma SA level before and 5, 15, 25 and 35 minutes after the injection. Statistical analysis of variance was employed to analyse the data. Results The plasma SA level was decreased significantly after the injection of both βEND and βEND antiserum and the time required to cause the decrease was shortened with the increase of βEND concentration. Conclusion Both endogenous and exogenous βEND play a role in the regulation process of decreasing rat plasma SA level.

关 键 词：唾液酸 Β内啡肽 侧脑室 抗血清 

分 类 号：R338[医药卫生—人体生理学]