骨髓来源细胞移植对糖尿病小鼠胰岛功能的影响  

Effect of bone marrow-derived cells transplantation on islets function of diabetic mice

作　　者：宋陆军[1] 高晓东[1] 沈坤堂[1] 王洪山[1] 牛伟新[1] 秦新裕[1] 

机构地区：[1]复旦大学附属中山医院普外科,上海200032

出　　处：《中华实验外科杂志》2008年第5期571-573,681,共4页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30371388）;上海市科学技术委员会重大资助项目（04DZl9505）

摘　　要：目的观察骨髓来源细胞（BMDCs）移植对糖尿病小鼠胰岛功能的影响。方法建立糖尿病小鼠模型并分成两组：实验组小鼠（n=8）通过尾静脉移植BMDCs；对照组小鼠（n=8）通过尾静脉注射磷酸盐缓冲液（PBS）。观察两组小鼠血糖的变化、胰岛数量、胰腺组织形态学特征及相关标记物的表达。结果与对照组比较，实验组小鼠移植后第4周血糖出现明显下降（20．7±5．2）比（27．1±1．4）mmol／L，P〈0．05，并持续下降至第6周（16．9±6．0）比（27．7±0．3）mmol／L，P〈0．01，胰岛数目显著增加（22．9±4．8）比（11．6±5．2）个，P〈0．01；实验组小鼠胰岛周围和胰岛内发现绿色荧光蛋白（GFP）阳性细胞，部分GFP阳性细胞同时表达CD34，但未发现同时表达GFP和insulin的细胞。结论BMDCs移植能促进糖尿病小鼠胰岛的修复和再生，但BMDCs在糖尿病小鼠体内不能转分化为胰岛β细胞，CD34阳性细胞在损伤胰岛修复和再生的过程中起重要作用。Objective To investigate the effect of bone marrow-derived cells （ BMDCs ） transplantation on islets function of diabetic mice. Methods Diabetic models in mice were established by multiply peritoneal injection of low-dose streptozotocin （STZ） and then divided into two groups： the BMDCs transplantation group （n = 8） and the control group （ n = 8 ）. Blood glucose was measured weekly after transplantation by glucometer. Histochemistry and immunofluorescence were performed to examine pancreatic histology, morphology and markers expressed in recipient pancreas. Results Compared with control mice,blood glucose levels in transplantationmice were significantly reduced at 4th weeks after transplantation [ （20.7 ±5.2） vs （27.1 ± 1.4） mmol/L,P 〈0.05] and maintained sustained to reduce at 6th week after transplantation [ （ 16.9 ± 6.0） vs （27.7 ± 0.3 ） mmol/L, P 〈 0.01 ]. The number of islets in BM- DCs transplanted mice was more than that in control mice [ （22.9 ± 4.8 ） vs （ 11.6 ± 5.2）, P 〈 0.01 ]. There were several GFP ± cells around islets and some cells simultaneously expressed CD34 in BMDCs transplanted mice. Conclusion BMDCs transplantation can repair injured islets and initiate pancreatic is-lets regeneration. CD34^＋ cells play a role in the process of islets repair and regeneration, but BMDCs can not differentiate into pancreatic 13 ceils in vivo.

关 键 词：骨髓移植 糖尿病 再生 

分 类 号：R587.2[医药卫生—内分泌]