脾脏在小鼠同种肝移植排斥反应中的作用  

Role of spleen in acute rejection of mice undergoing allogeneic liver transplantation

作　　者：万赤丹[1] 黄韬[1] 勾善淼[1] 周静[1] 刘涛[1] 王春友[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院普外科,武汉430022

出　　处：《中华实验外科杂志》2008年第5期604-605,682,共3页Chinese Journal of Experimental Surgery

摘　　要：目的观察脾切除在小鼠同种肝移植急性排斥反应过程中的作用。方法双袖套法建立小鼠原位肝移植模型，随机分为3组，即建模保留脾脏组、建模3d后切除脾脏与建模同时切除脾脏组，各组于移植术后14d处死，ELESA法测定血清IgM水平；肝功能检测采用速率法；流式细胞仪检测CD4与CD8T细胞亚群；并同时行肝脏及脾脏的病理形态观察。结果建模保留脾脏组、建模3d后切除脾脏与建模同时切除脾脏组血清IgM水平分别为3．0181±0．4627、3．0936±0．4559、3．1953±0．4449，各组间差异无统计学意义（P〉0．05）；ALT水平分别为108．6875±20．3657、83．0000±22．7799、76．8000±19．5784，差异有统计学意义（P〈0．05）；AST水平分别为：105．3750±29．0583、93．0000±22．7799、93．2000±33．4220，各组间差异无统计学意义（P〉0．05）；CD4’／CD8’T细胞分别为：1．9162±0．2778、1．5654±0．4750、1．4616±0．2762，差异有统计学意义（P〉0．05）；3组肝脏间质及汇管区淋巴细胞浸润程度依次减弱，供肝灶状坏死程度逐渐减轻，在保留脾脏组中建模后第14天脾脏边缘区及淋巴鞘较建模同时切除的脾脏增宽。结论在小鼠同种异体肝移植排斥反应中细胞免疫起主要作用，脾切除可部分抑制同种异体肝移植急性排斥反应，保护供体肝脏。Objective To study the role of spleen in acute rejection of mice undergoing allogeneie liver transplantation. Methods Orthotopie liver transplantation was performed from C57BL mice to KM mice by using a two-cuff techinique. C57BL mice as receptors were divided into 3 groups by simple random method ： Group A （ n = 18）, tranaplantlon without spleneetomy ; Group B （ n = 18 ）, transplantion and spleneetomy were done simultaneously;Group C （n = 18） ,spleneetomy was done 3 days after liver transplanta. tion. All mice were killed 14 days after liver transplantation. ALT, AST, IgM, subgroups of T cells, pathc, logical changes of lvier and kidney were analyzed. Results There was significant difference in ALT, subgroups of T cells between groups A and B （ P 〈 0.05） or groups A and C （ P 〈 0.05 ）, but there was no significant difference in IgM and AST among 3 groups （ P 〉 0.05 ）. The liver pathological changes in group A was more serious than in other two groups, and the marginal zone and lymphoid sheaths of spleen in group A were significantly wider than in other two groups. Conclusion Acute rejection induced by eellula immunity is the main portion of rejection in mice allogeneie liver transplantation; Spleeetomy can partly inhibit acute rejection and protect the transplanted liver,

关 键 词：肝移植 脾切除 急性排斥反应 

分 类 号：R657.3[医药卫生—外科学] R735.9[医药卫生—临床医学]