可溶性肿瘤坏死因子-α受体I重组腺病毒对大鼠肾缺血再灌注的治疗作用  

The observation of protectiive effects by injecting recombinant adenovirus encoded soluble TNF-α receptor-I into renal ischemia-reperfusion rats

作　　者：郑巍[1] 韩从辉[2] 詹鸣[1] 董秉政[2] 郝林[2] 郑克立[1] 

机构地区：[1]中山大学附属第五医院泌尿外科,珠海519000 [2]东南大学医学院附属徐州医院泌尿外科

出　　处：《中华实验外科杂志》2008年第5期633-634,共2页Chinese Journal of Experimental Surgery

基　　金：江苏省卫生厅基金（H200557）;广东省珠海市科委基金资助项目（20041051）;中国博士后基金资助项目（2003033429）

摘　　要：目的观察可溶性肿瘤坏死因子（TNF）-α受体I重组腺病毒（AdsTNFRI）过继阻断体内TNF-α的生物学活性，探讨其对大鼠肾缺血再灌注的治疗作用。方法雄性SD大鼠随机分成4组：假手术组、肾缺血再灌注模型组、肾缺血再灌注-sTNFRI组和肾缺血再灌注-Ad-LacZ组。缺血2h后，再灌注24h。取各组大鼠尾静脉血，制备血清。测定各组血清中TNF-α、sTNFRI、抗氧化酶T-SOD、CAT及脂质过氧化物丙二醛（MDA）水平。结果缺血再灌注大鼠体内抗氧化酶T-SOD、CAT较假手术组显著降低，TGF-α、MDA显著增高。可溶性TNF-α受体I重组腺病毒（Ad-sTNFRI）过继给缺血再灌注大鼠体内后，T-SOD、CAT、可溶性TNF-α受体I（sTNFR-I）显著增高，TGF-α、MDA显著降低。结论AdsTNFRI可中和缺血再灌注大鼠外周血中TNF-α水平，并可增强其抗氧化能力，降低MDA水平，抑制缺血再灌注大鼠体内氧化应激水平，对大鼠缺血再灌注有一定的治疗作用。Objective To explore the protectiive effects by injecting recombinant adenovirus encoded soluble TNF-α receptor-I into renal ischemia-reperfusion rats. Methods SD rats were randomly di- vided into 4 groups with 15 animals in each group. Group A：sham operation group;Group B：renal ischemi- a- reperfusion group ; Group C ： renal ischemia- reperfusion group with injection of recombinant adenovirus encoded soluble TNF-α receptor-I （sTNFR I ） gene;Group D：renal ischemia- reperfusion group with in- jection of recombinant adenovirus encoded LacZ gene. Rats in group B, group C and group D were reper- fused for 24 h,followed by 2 h ischemia. The serum was collected and the levels of TNF-α,sTNFR I ,total superoxide dismutase （T-SOD） ,catalase （CAT） and malonaldehyde （MDA） were determined. Results The levels of T-SOD and CAT in group C were lower than those in group A, and the levels of TGF-α and MDA were higher significantly （ P 〈 0.01 ）. The levels of T-SOD, CAT and sTNFR I were strikingly heightened when compared with those of group D, and the levels of TGF-α and MDA were declined markedly. Conclusion Recombinant adenovirus encoded soluble TNF-α receptor-I （AdsTNFR I ） could neutralize TGF-α,increase the capability of anti-oxidization, reduce the expression of MDA, and supress oxidative stress in renal isehemia-reperfusion rats.

关 键 词：再灌注损伤 TNF-Α 受体 腺病毒 氧化应激 

分 类 号：R692[医药卫生—泌尿科学]