小麦成熟胚离体培养研究  

Studies on in Vitro Culture of Mature Embryo of Wheat

作　　者：章忠贵[1] 李明浩[1] 史文娟[1] 王敏[1] 

机构地区：[1]安徽农业大学农学院,合肥230036

出　　处：《种子》2008年第4期17-21,共5页Seed

基　　金：安徽省教育厅自然科学重点项目(2006KJ055A);安徽农业大学大学生创新基金项目

摘　　要：以40种小麦基因型的成熟胚为外植体,采用MS诱导培养基和分化培养基,研究不同基因型间愈伤组织诱导的差异,以及低温预处理和不同光照条件对愈伤组织分化的影响。结果表明,不同基因型间出愈率差异显著,散射光照射和低温处理有利于愈伤组织的分化。同舟916、丰华8929、皖麦48、皖麦30具有较强的成熟胚离体培养力。The explants of mature embryo of 40 different wheat genotypes were cultured in MS inducing and differentiating media tO study the induction of the callus and effect on differentiation of the callus under low temperature pretreatment and light. The results indicated that distinct differences of induction frequency were significant among different genotypes. Low temperature pretreatment of mature embryo and scattering light on callus were propitious to induction and differentiation of the callus. Tongzhou 916, Fenghua 8929, Wanmai48 and Wanmai 30 had the best ability for vitro cukure of mature embryo among 40 wheat genotypes.

关 键 词：小麦 成熟胚 愈伤组织 低温处理 散射光 

分 类 号：S512.1[农业科学—作物学]