电针足三里穴对急性力竭运动大鼠胃肠动力和肌间神经丛一氧化氮合酶阳性神经元的影响  

The effects of electroacupuncture on gastrointestinal motility and nitric oxide synthase positive nerves in myenteric plexus in acute exhaustive exercise rats

作　　者：雷亚宁[1] 丁友梅[2] 

机构地区：[1]温州医学院组织学与胚胎学教研室,温州325035 [2]温州医学院测试中心,温州325035

出　　处：《中华物理医学与康复杂志》2008年第4期248-251,共4页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：湖北省自然科学基金项目（96J088）,温州市科技局科学研究计划项目（Y2004A114）

摘　　要：目的研究电针足三里穴对急性力竭运动大鼠胃肠动力和肌间神经丛一氧化氮合酶（NOS）阳性神经元的影响。方法将54只雄性Sprague-Dawley大鼠随机分成急性力竭运动组（运动组）、急性力竭运动＋电针组（电针组）和安静对照组（对照组）。运动组和电针组建立大鼠急性力竭运动模型，电针组采用电针刺激大鼠双侧足三里穴。测定胃排空和小肠推进速率，同时用酶组织化学方法和计算机图像分析技术对大鼠回肠肌间神经丛内NOS阳性神经元的数目和NOS的表达进行测定。结果与对照组相比，运动组大鼠胃内色素残留量明显增多（胃排空减慢），小肠推进速率明显降低，回肠肌间神经丛内NOS阳性神经元的数目和NOS的表达明显增高（P〈0．01）；与运动组相比，电针组大鼠胃内色素残留量显著降低（胃排空加快），小肠推进速率明显加快，回肠肌间神经丛内NOS阳性神经元的数目和NOS的表达明显下降（P〈0．01）。结论急性力竭运动大鼠肌间神经丛NOS阳性神经元的数目和NOS的表达增高可能是导致胃肠运动减慢的重要原因之一；电针作用能使急性力竭运动大鼠肌间神经丛NOS阳性神经元数目减少，下调NOS的表达，并促进其胃肠运动。Objective To study the effects of electroacupuncture （EA） on gastrointestinal motility and nitric oxide synthase （NOS） positive nerves in myenteric plexus in acute exhaustive exercise rats. Methods A total of 54 male Sprague-Dawley rats were randomly divided into an acute exhaustive exercise （AEE） group, an AEE plus EA group and a control （C） group. The gastric empty rate and intestinal motility rate were measured, and histological changes of NOS-positive nerves in ileum myenterie plexus were observed with enzymatic histoehemieal method and image analysis technique. Results In the rats of AEE group, the gastric empty rate and intestinal motility rate were delayed but the numbers of NOS-positive neurons and expression levels of NOS in the ileum myenterie plexus significantly increased comparing with those of the C group （P 〈 0.01 ）. Compared with AEE group, the gastric empty rate and intestinal motility rate were significantly increased but the number of NOS-positive neurons and expression level of NOS in the ileum myenterie plexus significantly decreased in AEE plus EA group （P 〈 0.01 ）. Conclusion The findings of this study suggest that increase of NOS-positive neurons and expression levels of NOS in the myenterie plexus is one of the mechanisms of slowing down of gastrointestinal movement after acute exhaustive exercise. Electroaeupuneture at Zusanli aeupoint may decrease the number of NOS-positive neurons and expression levels of NOS in the myenterie plexus, and improve gastrointestinal movement in acute exhaustive exercise rats.

关 键 词：电针 急性力竭运动 一氧化氮合酶 肌间神经丛 大鼠 

分 类 号：R245[医药卫生—针灸推拿学]