微生物对蛇足石杉扦插生根及若干生理生化指标的影响  

Effects of microbes on rooting and on several physiological and biochemical indexes of cuttings of Huperzia serrata

作　　者：李娜[1] 陈钧[1] 承曦[1] 朱大元[2] 

机构地区：[1]江苏大学药学院,江苏镇江212013 [2]中国科学院上海生命科学研究院上海药物研究所新药研究国家重点实验室,上海201023

出　　处：《中国中药杂志》2008年第8期873-877,共5页China Journal of Chinese Materia Medica

基　　金：中国科学院知识创新工程重大项目子课题(KSCX1-SW-11-6)

摘　　要：目的:研究原生境土壤微生物、内生真菌和根表真菌对蛇足石杉扦插生根率和若干生理生化指标的影响。方法:蛇足石杉分别在原生境土壤(土壤Ⅰ)、接种了内生真菌和根表真菌的灭菌土壤(土壤Ⅱ)、灭菌土壤(土壤Ⅲ)上进行扦插,调查蛇足石杉扦插生根率,测定可溶性蛋白含量,可溶性糖含量,PPO(多酚氧化酶),POD(过氧化物酶)活性、黄酮类化合物和石杉碱甲含量。结果:与土壤Ⅲ上的扦插蛇足石杉相比,土壤Ⅰ和土壤Ⅱ上植株生根率,分别提高了10%和16%,土壤Ⅱ上扦插植株茎中的可溶性蛋白含量显著提高(P<0.05),土壤Ⅰ上扦插植株叶片和茎中的可溶性糖含量显著提高(P<0.05),土壤Ⅰ和土壤Ⅱ上的扦插植株的黄酮类化合物含量显著提高。结论:原生境土壤微生物、内生真菌和根表真菌对扦插蛇足石杉的生根有促进作用,提高蛇足石杉体内的代谢水平。Objective： To study the effects of soil microbes of habitats, endophytic fungi and root surface fungi of Huperzia serrata on rooting rate of the cuttings and on several physiological and biochemical indexes. Method： The cuttings of H. serrata were planted on soil Ⅰ of primary habitat, the soil Ⅱ had been inoculated endophytic fungi and root surface fungi, the soil Ⅲ had been autoclaved, rooting rate was examined, and soluble protein and soluble sugar contents, activities of PPO and POD, flavonoids and huperzine A contents were determined. Result： Compared with the H. serrata that were planted on soil Ⅲ, soil Ⅰ and soil Ⅱ were found to increase the rooting rate by 10% and 16%, soil Ⅱ increased the soluble protein contents of stem of H. serrata （ P 〈 0. 05 ）, Soil Ⅰ increased the soluble sugar contents of leaves and stem of H. serrata （P 〈 0. 05 ）, soil I and Soil Ⅱ increased the flavonoids contents of H. serrata （ P 〈 0. 05 ）. Conclusion： Soil microbes from primary habitat, endophytic fungi and root surface fungi promote rooting, they also increase plant metabolism level of H. serrata.

关 键 词：蛇足石杉 扦插 生根 生理生化指标 

分 类 号：R282[医药卫生—中药学]