柴胡皂苷d抗肝纤维化大鼠脂质过氧化作用的研究  

Experimental study of saikosaponin-d(SSd) on lipid peroxidation of hepatic fibrosis on rat

作　　者：何燕[1] 胡志峰[1] 李平[1] 肖诚[1] 陈玉武[1] 李克明[1] 郭景珍[1] 潘琳[1] 熊佳鹏[1] 

机构地区：[1]卫生部中日友好医院,北京100029

出　　处：《中国中药杂志》2008年第8期915-919,共5页China Journal of Chinese Materia Medica

基　　金：国家重点基础研究发展计划(973)项目(2005CB523503);北京市自然科学基金项目(7052060)

摘　　要：目的:探讨柴胡皂苷d(SSd)对肝纤维化过程中脂质过氧化的影响。方法:采用腹腔注射二甲基亚硝胺(DMN)10 mg.kg-1诱导大鼠肝纤维化,以柴胡皂苷d(1.8 mg.kg-1)同时预防给药4周,检测正常组、模型组和SSd组大鼠血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、透明质酸(HA)、层粘连蛋白(LN)、IV型胶原(IV-C)及丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性及肝组织中MDA的含量和SOD的活性。结果:SSd能显著降低肝纤维化大鼠血清ALT,AST水平和HA,LN,IV-C的含量,并可提高肝组织中SOD的活性,降低血清和肝组织中的MDA的含量。结论:SSd具有很明显的保护肝细胞、抗肝纤维化的作用,其机制可能与抗脂质过氧化有关。Objective： To study the effect of SSd on lipid peroxidation during experimental hepatic fibrosis progression. Method： The experimental models of hepatic fibrosis were induced by intraperitoneal injection of dimethylnitrosamine （DMN） on rats. SSd was administered by intraperitoneal injection for 4 weeks. Serum was analyzed for alanine and aspartate aminotransferase （ ALT and AST）, hyaluronic acid （ HA）, laminin （ LN）, collagen IV （ IV-C）, malonaldehyde （MDA） and superoxide dismutase （SOD） activi- ties. Liver samples were measured for MDA contents and SOD activities in normal group, model group and SSd group. Result： SSd significantly decreased ALT and AST activities and lowered HA, LN and IV-C contents. It enhanced SOD activities in liver, while re- duced MDA contents both in serum and liver. Conclusion.. SSd has obvious effects of protecting hepatocytes and resisting hepatic fibro- sis, and the mechanism may be associated with its anti-lipid peroxidation effect.

关 键 词：柴胡皂苷D 肝纤维化 脂质过氧化 

分 类 号：R285[医药卫生—中药学]