云芝黄芪有效组分对荷瘤小鼠免疫功能的影响  

Immunoregulatory activity of polysaccharopeptide and astragalus polysaccharides on EAC Tumor-bearing mice

作　　者：李进[1] 鲍依稀[1] 祝绚[1] 刘靖 王宏萍[1] 

机构地区：[1]重庆医科大学免疫学教研室临床检验诊断学省部共建教育部重点实验室,重庆400016

出　　处：《中国中药杂志》2008年第8期924-927,共4页China Journal of Chinese Materia Medica

基　　金：重庆市科学技术委员会基金(CSTC;2006BB5039);重庆市教育委员会基金(KJ060307);重庆医科大学校办课题启动基金(QD200540)

摘　　要：目的:应用云芝黄芪有效组分单方及复方治疗肿瘤小鼠,以此来验证云芝黄芪有效组分单方及复方抗肿瘤的免疫调节活性。方法:建立艾氏腹水癌(EAC)实体型荷瘤小鼠动物模型,随机分为6组,每组10只:生理盐水组(NS,0.1 mL.kg-1.d-1)、阿霉素单纯化疗组(AMD,4 mg.kg-1.d-1,0.2 mL,连续3 d)、云芝糖肽组(PSP,250 mg.kg-1.d-1,0.2 mL)、黄芪多糖组(APS,250 mg.kg-1.d-1,0.2 mL)、云芝黄芪复方组(PSP+APS,250 mg.kg-1.d-1,0.1 mL)和阿霉素云芝黄芪综合治疗组(AMD+PSP+APS,用量同上)。分别治疗30 d后采用免疫细胞化学法检测荷瘤小鼠外周血T淋巴细胞亚群的变化情况,计算脏器指数、抑瘤率,并与对照组及化疗组进行比较。结果:云芝糖肽组和云芝黄芪复方组小鼠的CD3+,CD4+T细胞百分率以及CD4+/CD8+均显著高于对照组(P<0.05),CD8+T细胞的百分率较阿霉素化疗组显著降低(P<0.05);综合治疗组CD3+,CD4+T细胞百分率显著高于单纯化疗组(P<0.05);单纯化疗组小鼠胸腺指数较对照组明显降低(P<0.05),而综合治疗组的胸腺指数显著高于单纯化疗组(P<0.01);并且各给药组肿瘤质量均显著低于NS对照组(P<0.05)。结论:云芝糖肽、云芝黄芪复方对荷瘤小鼠及经化疗后的荷瘤小鼠均具有免疫增强作用。Objective：To investigate the immunoregnlatory activities of polysaccharopeptide and astragalus polysaccharides on EAC tumor-bearing mice. Method： Ehdich＇s ascites carcinoma （EAC） Kunming （KM） mice were used to establish the animal model for solid tumor. Mice were randomly divided into six groups （ n = 10 ） ： NS group （NS, 10 mL · kg^-1 · d^-1 ） , AMD group （AMD, 4 mg · kg^-1 · d^-1 , 0.2 mL, only for the first 3 days）, PSP group （PSP, 250 mg · kg^-1 · d^-1 ,0.2 mL）, APS group （APS, 250 mg · kg^-1 · d^-1 ,0.2 mL）, complex prescription group （PSP ＋ APS, 250 mg · kg^-1 · d^-1 ,0.1 mL） and combined treat group （AMD ＋ PSP ＋ APS, same dosage as above）. After thirty days of treatment, immunocytochemical method was employed to detect the changes of T-lymphocyte subsets in the PBMC of tumor-bearing mice. Subsequently, the organ indexes and tumor inhibition rate were calculated and compared with those of control group. Result ： Percentage of CD3^＋ , CD4^＋ T-cell and the ratio of CD4^＋/CD8^＋ were obviously prominence in the PSP and PSP ＋ APS groups compared with those of NS group （0.05） , percentage of CD8^＋ T-cell was significantly decreased compared with that of AMD group; percentage of CD3^＋ , CD4^＋ T-cell were obviously increased in AMD ＋PSP tAPS group relative to that of AMD group ; the thymus index of AMD group was significantly decreased compared with that of NS group, but the thymus index of AMD ＋ PSP ＋ APS group was obviously increased compared with that of AMD group; the weight of tumor in each administration group was significantly decreased compared with that of NS group. Conclusion： PSP and PSP ＋ APS complex prescription showed the remarkable immunoregnlation on EAC mice with chemotherapy or not.

关 键 词：云芝糖肽 黄芪多糖 艾氏腹水癌 淋巴细胞亚群 抑瘤率 脏器指数 

分 类 号：R285[医药卫生—中药学]