含淫羊藿总黄酮大鼠血清对成骨细胞发育的影响  

Effects of rat serum containing total flavonoid extract of Epimedium sagittatum on development of osteoblasts

作　　者：马慧萍[1] 贾正平[1] 陈克明[2] 张汝学[1] 李茂星[1] 王娟[1] 

机构地区：[1]兰州军区兰州总医院药材科,甘肃兰州730050 [2]兰州军区兰州总医院骨科研究所,甘肃兰州730050

出　　处：《中国中药杂志》2008年第8期928-931,共4页China Journal of Chinese Materia Medica

基　　金：全军“十五”规划重点项目(01Z008)

摘　　要：目的:研究含淫羊藿总黄酮大鼠血清对体外培养成骨细胞(ROB)增殖和分化的影响。方法:将淫羊藿总黄酮(TFE)以0.1,1,10,100μg.mL-14种质量浓度、含淫羊藿总黄酮大鼠血清(SRAT)以2.5%,5%,10%3种质量浓度分别加入新生大鼠颅骨成骨细胞培养液中,研究它们对细胞增殖和分化成熟的影响。细胞增殖采用MTT法进行分析,细胞分化是于培养第4,8,12,20天分别检测碱性磷酸酶活性(比色法)、骨钙素分泌量(放射免疫法)、钙盐沉积量(比色法)和矿化结节(组织化学法)等。结果:TFE 4种质量浓度均对ROB细胞增殖和分化无明显影响,2.5%和5%SRAT则表现出强烈的刺激细胞增殖活性并显著提高碱性磷酸酶活性、骨钙素分泌量、矿化结节数和钙盐沉积量。结论:淫羊藿总黄酮代谢产物强烈刺激成骨细胞的增殖并促进其分化成熟,说明淫羊藿总黄酮代谢产物具有抗骨质疏松的活性。Objective： To investigate the effects of total flavonoid extract of Epimedium sagittatum （TFE） on the proliferation and differentiation of newborn rat calvarial osteoblasts （ROB）. Method： TFE was supplemented into the culture medium of ROB at 0. 1, 1, 10 and 100μg · mL^-1 respectively. The serum of rats administered TFES （SRAT） was also added into the medium in a parallel treatment at 2. 5% , 5% and 10% respectively. Their effects on cell proliferation and differentiation was studied by MIT and the analysis of osteogenic differentiation marks. Result： TFE had no appreciable and on cell proliferation and differentiation at any concentration. However, 2. 5% and 5% SRAT stimulated cell proliferation strongly and, 5% SRAT significantly promoted the maturation and function of osteoblast by improving the alkaline phosphatase activity, osteocalcin secretion, calcium deposition and the number of mineralized nodular structures. Conclusion： The metabolites of TFE should be the anti-osteoporosis constitutes of Epimedium sagittatum.

关 键 词：淫羊藿 黄酮 成骨细胞 分化 增殖 

分 类 号：R285[医药卫生—中药学]