掌叶大黄悬浮培养细胞和根培养体系对鬼臼毒素的生物转化研究  

Biotransformation of podophyllotoxin by cell suspension culture and root culture of Rheum palmatum

作　　者：崔亚君[1] 刘晓峰[2] 韩健[2] 王宝荣[2] 果德安[2] 

机构地区：[1]上海中医药大学中药学院,上海201203 [2]北京大学药学院,北京100083

出　　处：《中国中药杂志》2008年第9期989-991,共3页China Journal of Chinese Materia Medica

摘　　要：目的:对鬼臼毒素进行生物转化研究。方法:利用已经建立的掌叶大黄细胞悬浮体系、根培养体系进行转化,色谱法分离化合物,波谱法鉴定转化产物的结构。结果:细胞悬浮体系使73.8%的鬼臼毒素发生了转化,根培养体系使56.3%的鬼臼毒素发生了转化。2种体系对鬼臼毒素的转化产物不同,前者转化生成鬼臼苦素,后者主要转化形成表鬼臼毒素和脱水鬼臼毒素。结论:鬼臼毒素对掌叶大黄细胞悬浮系统和根培养系统的pH无明显影响;2种掌叶大黄组织培养体系均能转化鬼臼毒素。Objective： To study the biotransformation of podophyilotoxin by the cell suspension culture and root culture systems of Rheum palmatum. Method： Using plant tissue culture technology and HPLC techniques to isolate products. The structures were elucidated by spectroscopic means. Result：Ceil suspension culture of R. palmatum could convert podophyilotoxin to produce picropodophyilotoxin with the yield of 73.8%, while root culture of R. palmatum could convert podophyilotoxin to produce epipodophyilotoxin and apopodophyllotoxin. Conclusion： Podophyilotoxin did not affect the pH value of the media used in tissue cultures. Both cell suspension culture and root culture of R. palmatum can convert podophyilotoxin.

关 键 词：鬼臼毒素 鬼臼苦素 表鬼臼毒素 生物转化 掌叶大黄 

分 类 号：S567.239[农业科学—中草药栽培]