天麻素对大鼠皮层缺氧神经细胞NR1亚基mRNA表达的影响  

Expression of NR1 mRNA of NMDA receptor by Gastrodine on hypoxia injury in cultured rat cerebral cortical neurons

作　　者：付蕾[1] 毛艳华[1] 高远[2] 刘蕾[1] 王志平[1] 李良辰[2] 

机构地区：[1]郑州大学药学院,河南郑州450001 [2]郑州大学基础医学院,河南郑州450001

出　　处：《中国中药杂志》2008年第9期1049-1052,共4页China Journal of Chinese Materia Medica

基　　金：河南省医学科技攻关项目(20050014)

摘　　要：目的:观察天麻素对体外培养大鼠皮层缺氧神经细胞N-甲基-D-天冬氨酸(NMDA)受体NR1亚基mRNA表达的影响,探讨天麻素在神经保护方面的可能作用机制。方法:体外培养大鼠胚胎皮层神经细胞,以缺氧无糖培养建立神经细胞损伤模型,采用不同质量浓度的天麻素(13,26,52 mg.L-1)处理细胞,用反转录聚合酶链式反应(RT-PCR)法检测神经细胞NR1 mRNA的表达。结果:神经细胞缺氧损伤后,NR1 mRNA的表达显著增高;加入不同质量浓度的天麻素后均能显著降低神经细胞NR1 mRNA的表达。其中以26,52 mg.L-1的抑制作用最佳。结论:天麻素可能通过拮抗由缺氧引起的神经细胞NMDA受体NR1亚基mRNA表达的增加,发挥其神经保护作用。Objective： To investigate the effect of gastrodine on the expression of NR1 mRNA of NMDA receptor in cultured rat cerebral cortical neurons injury induced by hypoxia. Method ： The injury models of cultured rat cerebral cortical neurons was made by hypoxia and hypoglucose . The concentration of 13,26, 52 mg · L^-1 of gastrodine was respectively added to the injured ceils. The RPCR technique was used to study the expression of NR1 mRNA of NMDA receptor. Result： The expression of NR1 mRNA increased markedly in hypoxia and hypoglucose injured cells, the increased expression can be weakened by gastrodine, more significant effect was found in the concentration of 26 mg · L^-1 和52 mg · L^-1. Conclusion： Gastrodine may act as a neuro -protector by weakening the expression of NR1 mRNA in cultured rat cerebral cortical neurons injury induced by hypoxia and hypoglucose.

关 键 词：天麻素 神经细胞 缺氧 NR1 mRNA 反转录聚合酶链式反应 

分 类 号：R285.5[医药卫生—中药学]