液相色谱-质谱联用法快速检测蔬菜水果中70种农药多残留分析的研究  

Study on the Analysis of 70 Pesticides in Fruits and Vegetables Using Liquid Chromatography with Tandem Mass Spectrometric Detection

作　　者：宋文华[1] 刘芳[1] 丁峰[1] 高敏苓[2] 王国峰[3] 

机构地区：[1]天津理工大学环境科学与安全工程学院,天津300191 [2]上海交通大学环境科学与工程学院,上海200240 [3]通标标准技术服务有限公司上海分公司生命科学部,上海200233

出　　处：《南开大学学报（自然科学版）》2008年第2期35-41,共7页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：国家自然科学基金(30771771);天津市自然科学基金(06YEJMJC05600)

摘　　要：建立了一种基于液相色谱一串联质谱法(LC-MS/MS)定量分析各种微量农药残留的方法,并应用于水果蔬菜中农药残留物的定量检测.实验采用乙腈提取水果蔬菜中的各种农药残留,应用固相萃取,经高效液相色谱分离,以串联质谱在多反应监测(MRM)模式下测定,在35min 内完成了70种农药的定量分析.70种农药在0.01～2.0μg/mL 内的线性关系良好(r>0.995),平均回收率为70%～120%,检出限为10μg/kg;该方法的检出限、回收率及相对标准偏差均能满足农药残留分析的要求.A multi-residue method for the determination of 70 pesticides in fruits and vegetables by means of LC-MS/MS is presented. The pesticides were extracted by acetonitrile,purified using SPE and finally detected by LC-MS/MS within 35 minutes. The method is proved to be linear obtaining correlation coefficients from 0. 995 to 1. 000 over the range 0. 01--2.00μg/mL. The obtained recoveries are in the range 70% -120 % and the LOD is 10 μg/kg. The LOD, recovery and RSD can meet the demands of the analysis of pesticide residue.

关 键 词：蔬菜水果 农药 多残留分析 液相色谱-质谱联用 

分 类 号：O657.63[理学—分析化学]