三种活血法对大鼠萎缩性胃炎癌前病变早期细胞凋亡的影响  

Effect of Three TCM Methods for Activating Blood Circulation on Early Stage Apoptosis in Rats with Chronic Atrophic Gastritis Complicated Precancerous Lesion

作　　者：张玉禄[1] 李军祥[1] 朱陵群[2] 崔巍[2] 

机构地区：[1]北京中医药大学东方医院消化科,北京100078 [2]北京中医药大学中医内科学教育部重点实验室

出　　处：《中国中西医结合杂志》2008年第5期448-450,共3页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.30572388)

摘　　要：目的研究活血化瘀法、理气活血法和益气活血法对大鼠慢性萎缩性胃炎(chronic atrophic gas-tritis,CAG)癌前病变早期细胞凋亡的影响。方法采用幽门弹簧插入配合高盐热淀粉糊灌胃法复制CAG癌前病变模型,用双标记流式细胞术分析3种活血法对CAG癌前病变大鼠早期细胞凋亡的影响。结果3种活血化瘀法干预12周后,CAG癌前病变自然恢复组大鼠早期细胞凋亡率明显高于空白对照组(P<0.01),3种活血法干预组早期细胞凋亡率均有降低,益气活血组、活血化瘀组、理气活血组与自然恢复组比较早期凋亡率的降低有显著性差异(P<0.01)。结论3种活血法对CAG癌前病变大鼠早期细胞凋亡均有抑制作用,其可能是活血法改善或逆转大鼠CAG癌前病变的作用机理之一。Objective To comparatively study the effects of three TCM methods for activating blood circulation, i.e. in combined with resolving stasis （ A）, regulating qi （B） and supplementing qi （C） , respectively, on early stage cell apoptosis in precancerous lesion of rats with chronic atrophic gastritis （CAG）. Methods Rat model of CAG with precancerous lesion was duplicated by insertion of spring in pylorus and gastric perfusion of high-salt hot paste; and the impact of treatment on cell apoptosis was determined using Annexin V/PI double labeled flow cytometry. Results After being intervened for 12 weeks, the early stage cell apoptosis rate in the natural recovery group was significantly higher than that in the control group （P 〈0. 01 ） ; while it lowered more significantly in the three groups receiving TCM therapeutic methods for activating blood circulation, showing significant difference compared with the natural recovery group （P 〈0.01 ）. Conclusion Three therapies of activating blood circulation all show inhibitory action on the early stage apoptosis of precancerous lesion in CAG rats, which is possibly one of their action mechanisms for improving or reversing the precancerous lesion.

关 键 词：萎缩性胃炎 癌前病变 活血法 流式细胞术 大鼠 

分 类 号：R259[医药卫生—中西医结合]