高脂血症对孕期大鼠急性胰腺炎的影响  

Influence of Hyperlipemia on Rats with Acute Pancreatitis During Pregnancy

作　　者：刘兴木[1] 文军[1] 黎永胜[1] 杨少华[1] 陈少逸[1] 沈文律[1] 

机构地区：[1]汕头大学医学院第二附属医院普外科,汕头515041

出　　处：《中国普外基础与临床杂志》2008年第5期342-346,共5页Chinese Journal of Bases and Clinics In General Surgery

摘　　要：目的研究高脂血症对孕期大鼠急性胰腺炎发生的影响,并探讨其可能机理。方法72只妊娠大鼠按抽签法分为高脂饮食组和均衡饮食组,分别喂养16d后,用L-精氨酸盐酸盐溶液(250mg/100g)进行腹腔内注射,1h后再次腹腔内注射(200mg/100g)。分别于末次注射后0、12、18、24、30和48h各取6只大鼠检测血清甘油三酯、淀粉酶和脂肪酶,计算胰腺湿/干重比值,观察胰腺组织的病理变化并评分,免疫组化SP法检测胰腺组织TNF-α表达。结果各时相高脂饮食组甘油三酯均较均衡饮食组明显升高(P<0.05);高脂饮食组淀粉酶及脂肪酶的峰值分别在24h及18h出现,均衡饮食组延至30及24h时出现且明显较高脂饮食组水平低(P<0.05);在L-精氨酸盐酸盐溶液末次注射后12、18和24h,高脂饮食组的胰腺湿/干重比值较均衡饮食组明显升高(P<0.05),TNF-α表达也更高(P<0.05);在0、12、18及24h,高脂饮食组胰腺水肿出血、炎症浸润更重,病理评分更高(P<0.05)。结论高脂血症能使L-精氨酸诱导的孕期急性胰腺炎提前出现,后果更严重,可能是胰腺炎发生、发展的一个危险因素,其机理可能是高脂血症时甘油三酯大量释放游离脂肪酸,上调TNF-α的表达。Objective To study the influence of hyperlipemia on rats with acute pancreatits during pregnancy and its mechanism. Methods Seventy two pregnant Sprague-Dawley rats were randomly divided into the test group and the control group, and then they were fed with high fat diet and balanced diet for 16 days separately. Pregnant rats were given intraperitoneal injection with L-arginine for 2 times （one time is 250 mg/100 g, the other is 200 mg/100 g） at an interval of 1 h. The serum triglyceride （TG）, serum amylase （AMS）, and lipase （LPS） from blood samples were tested just after injection, and 12 h, 18 h, 24 h, 30 h and 48 h after injection respectively, and wet/dry ratio of pancreas were measured. The histopathological score of pancreatic tissue was evaluated based on microscopic changes, and the expression of TNF-a protein was determined by SP immunohistochemical technique. Results After the last injection, the level of TG in the test group was obviously higher than that in the control group in each time （P〈0.05）. The peak values of AMS and LPS in the test group appeared at 24, 18 h respectively, while the peaks appeared at 30, 24 h in the control group, respectively, which were significantly lower than those in the test group （P〈0.05）. Compared with the control group, the wet/dry ratio of pancreas in the test group increased at 12, 18 and 24 h after injection （P〈0.05）; The pathological changes of pancreas in test group was more serious with higher histopathological score at 0, 12, 18 and 24 h （P〈0.05）, and expression of the TNF-α protein was higher at 12, 18 and 24 h （P〈0.05）, too. Conclusion Hyperlipemia can make L-arginine-induced-acute-pancreatitis during pregnancy earlier occur and lead to more serious injury in pancreas. This study demonstrates that hyperlipemia may be a high risk factor for acute pancreatitis during pregnancy, making a great amount of free fatty acid released from TG and up-regulated the expression of TNF-α.

关 键 词：高脂血症 孕期 急性胰腺炎 L-精氨酸 

分 类 号：R714.2[医药卫生—妇产科学] R589.2[医药卫生—临床医学]