放射损伤对恶性肿瘤外周血CD4^+CD25^+调节性T细胞的影响  

Influence of radiotherapy on CD4^+CD25^+ regulatory cells in peripheral blood of cancer patients

作　　者：王娟[1] 杨朝群[2] 

机构地区：[1]石河子大学医学院 [2]石河子大学医学院第一附属医院放疗科,新疆石河子832002

出　　处：《农垦医学》2008年第2期84-86,共3页Journal of Nongken Medicine

摘　　要：目的:观察放射治疗过程中不同剂量时患者外周血T细胞亚群和CD4+CD25+调节性T细胞的变化。并探讨其临床意义。方法:用流式细胞仪检测放射治疗中不同剂量时患者外周血T细胞亚群和CD4+CD25+调节性T细胞的水平,并进行分层分析。结果:放疗剂量为30GY、50GY时,恶性肿瘤患者CD3+、CD4+、CD8+T细胞比例及CD4+/CD8+比值与放疗前相比,差异无统计学意义,而早期(II、I)、进展期(IIII、V)患者外周血CD4+CD25+调节性T细胞的水平与放疗前相比,差异有统计学意义(F=4.66,P=0.041),且与剂量呈正相关。结论:放疗可能对恶性肿瘤患者的免疫功能有一定的损害,尤其是患者外周血CD4+CD25+调节性T细胞水平升高,且它与剂量呈正相关;放疗后外周血CD4+CD25+调节性T细胞比例比CD4+/CD8+比值更能敏感反应免疫功能状态,可能成为恶性肿瘤患者的更敏感的免疫监测指标。Objective： The current study was designed to investigate the changes in peripheral CD4^ ＋ CD25^ ＋ regulatory cells in cancer patients and the influence of different radiotherapy dose on immunity function. Methods： The peripheral blood was collected from 31 patients with cancer patients. By using monoclonal antibodies, the blood samples were evaluated with flow cytometry for T cell subsets and CD4^ ＋ CD25^ ＋ regulatory cells. Results：The levels of peripheral blood T cell subsets and CD4^ ＋ CD25^ ＋ regulatory cells are detected when different radiotherapy dose（30GY, 50GY）. The ratio of CD4^ ＋ CD8^ ＋ in peripheral blood of cancer patients was not significantly less than before it, but the level of CD4^ ＋ CD25^ ＋ regulatory cells was higher than before it（ P 〈 0. 05 ）, There was potisive relation between the level of CD4^ ＋ CD25^ ＋ regulatory cells and radiotherapy does. Conclusions： The radiotherapy may be reduce the immune function of cancer patients, especially expression of CD4^ ＋ CD25^ ＋ regulatory cells, This finding suggests that CD4^ ＋ CD25 ^＋ regulatory cells in peripheral blood of cancer patients can be a useful index for monitoring the immunity function.

关 键 词：放疗 不同剂量 T细胞亚群 CD4^+CD25^+调节性T细胞 流式细胞仪 

分 类 号：R815[医药卫生—放射医学] R392.1[医药卫生—临床医学]