珍稀濒危植物香果树胚性细胞悬浮系的建立和植株再生  

Establishment of Embryogenic Cell Suspension Culture and Plant Regeneration of Rare Endangered Plant Emmenopterys henryi

作　　者：熊丹[1] 陈发菊[1,2] 梁宏伟[1] 王玉兵[1] 

机构地区：[1]三峡大学生物技术研究中心,湖北省天然产物研究与利用重点实验室,宜昌443002 [2]湖北民族学院湖北省生物资源保护与利用重点实验室,恩施445000

出　　处：《植物学通报》2008年第3期337-343,共7页Chinese Bulletin of Botany

基　　金：湖北省生物资源保护与利用重点实验室开放基金(No.2007010);国家林业局自然保护区研究中心项目(No.460-8101);宜昌市科技局重点攻关项目(No.A06209)

摘　　要：以香果树(Emmenopteryshenryi)未成熟种子为试材,探讨不同的接种量、基本培养基、糖浓度及植物生长调节物质等对体细胞胚胎生长的影响,建立稳定的香果树胚性细胞悬浮培养与植株再生体系。结果表明:悬浮培养条件下,最适接种量为2%(鲜重);较适合的基本培养基为MS;蔗糖浓度为1%时容易使球形胚聚合体愈伤化,浓度为3%和6%适合球形胚聚合体增殖,浓度为9%则容易使球形胚聚合体褐化;添加0.5mg.L^(-1)6-BA和0.5mg.L^(-1)NAA的MS液体培养基,当初始蔗糖浓度为3%,然后逐步提高到6%则有利于香果树各个发育阶段的同步化;子叶胚转到不含任何植物生长调节物质的MS固体培养基中可以长成正常植株。Embryogenic cell suspension cultures were established from calli of immature seeds of Emmenopterys henryi. Callus could be obtained from immature seeds on MS solid medium augmented with 2.0 mg.L^-1 6-benzylaminopurine （6-BA） and 3% sucrose. Embryogenic calli were induced on MS basal medium supplemented with 0.1 mg.L^-1 N-acetyl aspartate （NAA） and 0.5 mg.L^-1 6-BA. Numerous somatic embryoids appeared on MS liquid basal nutrient medium with 0.5 mg.L^-1 NAA and 0.5 mg.L^-1 6-BA. The optimal value of the initial cell density was 2.0% （fresh weight）. Several parameters that might be associated with growth were determined over a 14-day period. The embryo-derived plantlets with well-developed roots and shoots were transferred successfully to the greenhouse, with a high survival rate of 95%.

关 键 词：胚性悬浮细胞 香果树 植株再生 体细胞胚胎发生 

分 类 号：Q943.1[生物学—植物学]