丹参对体外关节软骨细胞抗过氧化氢损伤实验研究  

Effect of salvia miltiorrhiza on injury of cartilage cells in vitro induced by hydrogen peroxide

作　　者：程义权[1] 郭振河[1] 杨家辉[1] 余伦红[1] 

机构地区：[1]解放军第161中心医院骨科,湖北武汉430010

出　　处：《重庆医学》2008年第10期1030-1032,F0003,共4页Chongqing medicine

摘　　要：目的观察过氧化氢(H2O2)对体外培养关节软骨细胞的损伤效应及丹参(salvia miltiorrhiza,SM)的干预作用。方法兔原代膝关节软骨细胞培养,Ⅱ型胶原蛋白免疫荧光检测鉴定。将培养的软骨细胞随机分为正常组、损伤对照组(损伤组)和丹参组。损伤组用0.1mmol/L过氧化氢(终浓度)制造体外软骨细胞损伤模型,丹参组将不同浓度的丹参(终浓度0.01g/L、0.05g/L、0.25g/L)于过氧化氢损伤前30min加入,分别测定各组细胞活力(MTT法)、培养液上清中丙二醛(MDA)含量(硫代巴比妥酸法)、总蛋白含量(考马斯亮蓝法)及细胞DNA合成计数(氚标记脱氧嘧啶核苷酸法,3H-TdR法)。结果损伤组细胞活力显著降低、培养液上清MDA含量显著上升、蛋白含量及细胞DNA合成显著下降。丹参组细胞活性较损伤组显著升高、培养液上清MDA水平显著降低、蛋白含量及细胞DNA合成显著上升。结论丹参能抵抗过氧化氢造成的体外软骨细胞损伤。Objective To observe the injury of hydrogen peroxide on the cartilage cells cultured from joint and the effect of salvia miltiorrhiza on the cartilage cells. Methods Rabbit chondrocytes were primary cultured and identified by fluorescence detection of Ⅱ type collagen protein. There were three groups, the normal group, the injury control group and the experiment group. In the control group, hydrogen peroxide of 0.1 mmol/L was used. In the experiment group, salvia mihiorrhiza of different content （0. 05g/L,0.05g/ L,0.25g/ L ）were used before the usage of hydrogen peroxide. The proliferation of cartilage cell was detected by MTT methods. The content of MDA in the supernatant, protein level and DNA synthesis（3 H-TdR method） were observed too. Results Hydrogen peroxide could damage cartilage cells in vitro. Compared with the injury control group, the proliferation of cartilage cells in the experiment group was higher. The content of MDA in the supernatant decreased. The protein level and DNA synthesis were higher. Conclusion Salvia mihiorrhiza can resist the injury of hydrogen peroxide on cartilage cells in vitro.

关 键 词：骨性关节炎 软骨细胞 丹参 过氧化氢 

分 类 号：R285[医药卫生—中药学]