10个绵羊品种的微卫星DNA多态性研究  

Genetic Diversity of Microsatellite DNA among Ten Sheep Breeds

作　　者：仲涛[1] 马月辉[1] 关伟军[1] 凌英会[1] 郭军[1] 赵倩君[1] 何晓红[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所

出　　处：《畜牧兽医学报》2008年第5期555-561,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：“十一五”科技支撑计划(2006BAD13B08);国家自然科学基金(30371026);国际原子能机构研究项目(130071R0)资助

摘　　要：本研究利用FAO和ILRI推荐的21个微卫星引物，结合荧光标记PCR，检测了中国9个地方绵羊（Ovisaries）品种和1个外来绵羊品种的遗传多样性。21个微卫星座位均呈现出高度多态，多态性信息含量和杂合度均表明我国地方绵羊品种有着丰富的遗传多样性。本研究共检测到342个等位基因，有效等位基因数在2．1752～9．4997之间，座位平均杂合度在0．5248～0．8551之间，品种平均杂合度在0．633-0．761之间。聚类关系和主成分分析结果与其起源、育成历史及地理分布基本一致。In order to investigate the genetic diversity of Chinese indigenous sheep breeds, genetic variation of 9 Chinese indigenous sheep breeds and one introduced breed were examined using 21 microsatellite DNA markers and fluorescence PCR. All the 21 markers are highly polymorphic. The polymorphism information content （PIC） and genetic heterozygosis showed that the Chinese local breeds have high genetic diversity. A total of 342 alleles were detected, and the effective al- lele number ranged from 2. 175 2 to 9. 499 7. The average heterozygosity of loci varied from 0. 524 8 to 0. 855 1, and the average heterozygosity of breeds were from 0. 633 to 0. 761. The phylogenetic trees（NJ and UPGMA） analysis were generally in accordance with their origins, breeding history and localities.

关 键 词：绵羊 遗传多样性 遗传距离 聚类关系 

分 类 号：S826.2[农业科学—畜牧学]